Regional levels of phospholipase Cgamma after fluid percussion brain injury in the rat.

H M Carman, H S Dhillon, D Zhang, J W Geddes, R M Prasad
Author Information
  1. H M Carman: Department of Surgery, University of Kentucky Chandler Medical Center, Lexington, KY 40536-0084, USA.

Abstract

Levels of PLCgamma, a phospholipase C (PLC) isozyme, were significantly increased in the cytosol in the injured left cortex (LC) at 5, 30 and 120 min after brain injury. In the same site, although levels of membrane PLCgamma did not alter at 5 and 30 min, they were found to be decreased at 2 h after brain injury. In general, the levels of both cytosolic and membrane PLCgamma were unaltered in the contralateral right cortex (RC), ipsilateral left hippocampus (LH) and contralateral right hippocampus (RH) between 5 and 120 min after brain injury. These results suggest that, in addition to well-proposed excitatory neurotransmitter-receptor systems, increased levels of PLCgamma may also contribute to alterations in PIP2 signal transduction pathway, particularly in the greatest injury site (LC) after lateral FP brain injury.

Grants

  1. NS 34264/NINDS NIH HHS

MeSH Term

Animals
Brain
Brain Injuries
Cell Membrane
Cerebral Cortex
Cytosol
Functional Laterality
Hippocampus
Isoenzymes
Male
Organ Specificity
Percussion
Phospholipase C gamma
Rats
Rats, Sprague-Dawley
Reference Values
Time Factors
Type C Phospholipases

Chemicals

Isoenzymes
Type C Phospholipases
Phospholipase C gamma

Word Cloud

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