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The Journal of neuroscience : the official journal of the Society for Neuroscience
Jul 15, 2001;21 (14): 4996-5006.

FGF induces a switch in death receptor pathways in neuronal cells.

E M Eves, C Skoczylas, K Yoshida, E S Alnemri, M R Rosner
Author Information
  1. E M Eves: Ben May Institute for Cancer Research, Department of Neurobiology, University of Chicago, Chicago, Illinois 60637, USA.
PMID: 11438575

Abstract

Basic fibroblast growth factor (FGF2) has many roles in neuronal development and maintenance including effects on mitogenesis, survival, fate determination, differentiation, and migration. Using a conditionally immortalized rat hippocampal cell line, H19-7, and primary hippocampal cultures, we now demonstrate that FGF2 treatment differentially regulates members of the tumor necrosis factor (TNF) superfamily of death domain receptors and their ligands. H19-7 cells transferred from serum to defined (N2) medium undergo apoptosis by a Fas-dependent mechanism similar to primary neurons. In contrast, H19-7 cells treated with FGF undergo apoptosis by a Fas-independent mechanism. FGF suppresses the Fas death pathway but also induces apoptosis by activation of a TNFalpha death pathway in both H19-7 and hippocampal progenitor cells. Expression of the TNF receptor 1 (TNFR1) or TNFR2 in H19-7 cells is sufficient to sensitize the cells to TNFalpha, similar to the effects of FGF. Because TNFalpha can be either proapoptotic or antiapoptotic, these results provide an explanation for the divergent trophic effects of FGF2 treatment and the observation that multiple trophic inputs are required for the survival of specific neurons.

References

  1. Brain Res. 1977 May 13;126(3):397-42 [PMID: 861729]
  2. J Neurosci. 1995 Oct;15(10):6666-78 [PMID: 7472427]
  3. Biochem Biophys Res Commun. 2000 Jun 16;272(3):936-45 [PMID: 10860854]
  4. Cell. 1997 Aug 8;90(3):405-13 [PMID: 9267021]
  5. Neurosci Biobehav Rev. 1997 Sep;21(5):603-13 [PMID: 9353794]
  6. Mol Cell Biol. 2000 Aug;20(15):5392-403 [PMID: 10891480]
  7. Neuron. 1995 Sep;15(3):649-61 [PMID: 7546744]
  8. Nature. 1970 Aug 15;227(5259):680-5 [PMID: 5432063]
  9. J Neurochem. 1996 Nov;67(5):1908-20 [PMID: 8863495]
  10. Neuron. 1999 Apr;22(4):667-76 [PMID: 10230788]
  11. Cell. 1998 Aug 7;94(3):325-37 [PMID: 9708735]
  12. Mol Cell Neurosci. 1995 Oct;6(5):474-86 [PMID: 8581317]
  13. Mol Cell Biol. 1999 Jan;19(1):751-63 [PMID: 9858598]
  14. J Biol Chem. 1999 Mar 26;274(13):8531-8 [PMID: 10085086]
  15. J Neurosci. 1999 Mar 1;19(5):1754-70 [PMID: 10024361]
  16. Cell. 1997 Nov 14;91(4):479-89 [PMID: 9390557]
  17. Cell. 1998 Aug 21;94(4):481-90 [PMID: 9727491]
  18. Brain Res. 1988 Jun 7;451(1-2):205-12 [PMID: 2472189]
  19. Cell. 1999 Mar 19;96(6):857-68 [PMID: 10102273]
  20. EMBO J. 1997 May 1;16(9):2271-81 [PMID: 9171342]
  21. Science. 1998 Sep 18;281(5384):1860-3 [PMID: 9743501]
  22. Mol Cell Biol. 1998 Apr;18(4):2143-52 [PMID: 9528786]
  23. J Immunol. 1992 Dec 15;149(12):3894-902 [PMID: 1460280]
  24. J Biol Chem. 1997 Jul 11;272(28):17255-7 [PMID: 9211860]
  25. Mech Dev. 1996 Sep;59(1):89-102 [PMID: 8892235]
  26. J Biol Chem. 1993 Jul 25;268(21):15536-43 [PMID: 8393447]
  27. Exp Hematol. 1999 Mar;27(3):512-9 [PMID: 10089914]
  28. Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11333-8 [PMID: 9326610]
  29. EMBO J. 1997 Apr 1;16(7):1686-94 [PMID: 9130713]
  30. Genes Dev. 1996 Dec 15;10(24):3129-40 [PMID: 8985182]
  31. Life Sci. 1998;62(10):889-96 [PMID: 9496711]
  32. Biochem Pharmacol. 1998 Oct 15;56(8):915-20 [PMID: 9776301]
  33. Neuron. 1998 Apr;20(4):633-47 [PMID: 9581757]
  34. Immunity. 1997 Jun;6(6):751-63 [PMID: 9208847]
  35. J Neurosci. 1995 Aug;15(8):5851-7 [PMID: 7643225]
  36. Biochem Biophys Res Commun. 1998 Nov 27;252(3):623-8 [PMID: 9837756]
  37. Brain Res Mol Brain Res. 1998 Nov 20;62(2):111-21 [PMID: 9813268]
  38. Exp Cell Res. 2000 Apr 10;256(1):50-7 [PMID: 10739651]
  39. Annu Rev Biochem. 1999;68:383-424 [PMID: 10872455]
  40. Mol Cell Biol. 1998 Apr;18(4):2272-81 [PMID: 9528798]
  41. Jpn J Pharmacol. 1990 Sep;54(1):45-51 [PMID: 2273647]
  42. Curr Opin Neurobiol. 1994 Feb;4(1):78-86 [PMID: 8173328]
  43. Nature. 1997 Jul 10;388(6638):190-5 [PMID: 9217161]
  44. Neuron. 1995 Jul;15(1):105-14 [PMID: 7619514]
  45. Toxicol Appl Pharmacol. 1998 Jun;150(2):271-6 [PMID: 9653057]
  46. J Immunol. 2000 Jun 15;164(12):6349-58 [PMID: 10843689]
  47. Trends Neurosci. 2000 Jul;23(7):291-7 [PMID: 10856938]
  48. Brain Res. 1994 Sep 12;656(2):396-404 [PMID: 7820600]
  49. J Rheumatol. 1999 Mar;26(3):645-53 [PMID: 10090177]
  50. J Biol Chem. 1997 Jul 25;272(30):18542-5 [PMID: 9228018]
  51. Mol Cell Biol. 1999 Jun;19(6):4209-18 [PMID: 10330161]
  52. Annu Rev Neurosci. 1991;14:453-501 [PMID: 2031577]
  53. J Neurochem. 1999 Aug;73(2):612-22 [PMID: 10428057]
  54. Brain Res. 1988 May 1;468(1):71-9 [PMID: 3132309]
  55. Science. 1999 Jan 22;283(5401):543-6 [PMID: 9915703]
  56. Proc Natl Acad Sci U S A. 1996 May 28;93(11):5635-40 [PMID: 8643629]
  57. Adv Neurol. 1997;72:113-9 [PMID: 8993690]
  58. J Cell Biol. 1999 Nov 29;147(5):1049-62 [PMID: 10579724]
  59. Brain Res Mol Brain Res. 1996 Jun;38(2):222-32 [PMID: 8793110]
  60. Proc Natl Acad Sci U S A. 1992 May 15;89(10):4373-7 [PMID: 1316607]
  61. EMBO J. 1998 Aug 3;17(15):4213-25 [PMID: 9687490]
  62. Cell. 1998 Aug 21;94(4):491-501 [PMID: 9727492]
  63. Genes Dev. 1995 Sep 1;9(17):2157-69 [PMID: 7657167]
  64. Neuron. 1993 Oct;11(4):621-32 [PMID: 8398151]
  65. J Neurosci. 1999 Oct 1;19(19):8487-97 [PMID: 10493749]
  66. J Biol Chem. 1999 Sep 10;274(37):26287-95 [PMID: 10473584]
  67. Mol Cell Biol. 2000 Aug;20(16):6084-94 [PMID: 10913190]

Grants

  1. NS33858/NINDS NIH HHS

MeSH Term

Animals
Antigens, CD
Apoptosis
CASP8 and FADD-Like Apoptosis Regulating Protein
Carrier Proteins
Caspase Inhibitors
Caspases
Cell Survival
Cells, Cultured
Dose-Response Relationship, Drug
Fas Ligand Protein
Fibroblast Growth Factor 2
Hippocampus
Intracellular Signaling Peptides and Proteins
Membrane Glycoproteins
Neurons
RNA, Messenger
Rats
Receptors, Tumor Necrosis Factor
Receptors, Tumor Necrosis Factor, Type I
Receptors, Tumor Necrosis Factor, Type II
Signal Transduction
Transfection
Tumor Necrosis Factor-alpha
fas Receptor

Chemicals

Antigens, CD
CASP8 and FADD-Like Apoptosis Regulating Protein
Carrier Proteins
Caspase Inhibitors
Fas Ligand Protein
Faslg protein, rat
Intracellular Signaling Peptides and Proteins
Membrane Glycoproteins
RNA, Messenger
Receptors, Tumor Necrosis Factor
Receptors, Tumor Necrosis Factor, Type I
Receptors, Tumor Necrosis Factor, Type II
Tumor Necrosis Factor-alpha
fas Receptor
Fibroblast Growth Factor 2
Caspases
Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Word Cloud

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