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Genes & development
Jan 01, 2002;16 (1): 85-100.

Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae.

Judith A Sharp, Alexa A Franco, Mary Ann Osley, Paul D Kaufman
Author Information
  1. Judith A Sharp: Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
PMID: 11782447 DOI: 10.1101/gad.925302

Abstract

Budding yeast centromeres are comprised of approximately 125-bp DNA sequences that direct formation of the kinetochore, a specialized chromatin structure that mediates spindle attachment to chromosomes. We report here a novel role for the histone deposition complex chromatin assembly factor I (CAF-I) in building centromeric chromatin. The contribution of CAF-I to kinetochore function overlaps that of the Hir proteins, which have also been implicated in nucleosome formation and heterochromatic gene silencing. cacDelta hirDelta double mutant cells lacking both CAF-I and Hir proteins are delayed in anaphase entry in a spindle assembly checkpoint-dependent manner. Further, cacDelta and hirDelta deletions together cause increased rates of chromosome missegregation, genetic synergies with mutations in kinetochore protein genes, and alterations in centromeric chromatin structure. Finally, CAF-I subunits and Hir1 are enriched at centromeres, indicating that these proteins make a direct contribution to centromeric chromatin structures.

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Grants

  1. R01 GM040118/NIGMS NIH HHS
  2. R01 GM055712/NIGMS NIH HHS
  3. 1 R01 GM55712/NIGMS NIH HHS
  4. GM40118/NIGMS NIH HHS

MeSH Term

Chromatin Assembly Factor-1
Chromosomal Proteins, Non-Histone
Chromosome Segregation
DNA-Binding Proteins
Kinetochores
Mitosis
Mutation
Nuclear Proteins
Saccharomyces cerevisiae

Chemicals

Chromatin Assembly Factor-1
Chromosomal Proteins, Non-Histone
DNA-Binding Proteins
Nuclear Proteins
Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.
Article has an altmetric score of 4

Word Cloud

Created with Highcharts 10.0.0chromatinCAF-IproteinskinetochoreassemblycentromericHircentromeresdirectformationstructurespindlefactorbuildingcontributioncacDeltahirDeltaBuddingyeastcomprisedapproximately125-bpDNAsequencesspecializedmediatesattachmentchromosomesreportnovelrolehistonedepositioncomplexfunctionoverlapsalsoimplicatednucleosomeheterochromaticgenesilencingdoublemutantcellslackingdelayedanaphaseentrycheckpoint-dependentmannerdeletionstogethercauseincreasedrateschromosomemissegregationgeneticsynergiesmutationsproteingenesalterationsFinallysubunitsHir1enrichedindicatingmakestructuresChromatincontributefunctionalkinetochoresScerevisiae

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