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Journal of biosciences
Jun, 2002;27 (3): 261-72.

Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy.

T Venugopal, S Mathavan, T J Pandian
Author Information
  1. T Venugopal: Center for Advanced Studies in Functional Genomics, School of Biological Sciences, Madurai Kamaraj University, Madurai 625 021, India.
PMID: 12089475 DOI: 10.1007/BF02704915

Abstract

A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences of Labeo rohita, Cirrhina mrigala and Catla catla have been cloned, characterized and overexpressed in Escherichia coli. These sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.

References

  1. Mol Cell Biol. 1982 Feb;2(2):161-70 [PMID: 6287227]
  2. Gen Comp Endocrinol. 1991 Oct;84(1):135-46 [PMID: 1778403]
  3. Nucleic Acids Res. 1996 Feb 15;24(4):795-6 [PMID: 8604326]
  4. Nature. 1970 Aug 15;227(5259):680-5 [PMID: 5432063]
  5. Trends Genet. 1993 Mar;9(3):70-1 [PMID: 8488563]
  6. Bioinformatics. 1999 May;15(5):413-21 [PMID: 10366661]
  7. Biochemistry. 1991 May 7;30(18):4389-98 [PMID: 2021631]
  8. Gene. 1983 Nov;25(2-3):263-9 [PMID: 6198242]
  9. Gen Comp Endocrinol. 1990 Aug;79(2):233-9 [PMID: 1697273]
  10. J Mol Evol. 1993 Dec;37(6):644-9 [PMID: 8114117]
  11. Fish Physiol Biochem. 1993 Jul;11(1-6):155-64 [PMID: 24202472]
  12. Mol Mar Biol Biotechnol. 1991 Sep;1(1):73-7 [PMID: 1845473]
  13. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9343-7 [PMID: 3467309]
  14. Protein Eng. 1990 May;3(6):509-14 [PMID: 2371258]
  15. Fish Physiol Biochem. 1993 Jul;11(1-6):363-9 [PMID: 24202496]
  16. Science. 1992 Jan 17;255(5042):306-12 [PMID: 1549776]
  17. Endocr Rev. 1983 Spring;4(2):97-130 [PMID: 6345149]
  18. J Biol Chem. 1989 Jan 5;264(1):312-6 [PMID: 2909523]
  19. Bioessays. 1990 Mar;12(3):121-4 [PMID: 2182006]
  20. Biochemistry. 1978 Feb 7;17(3):550-6 [PMID: 620007]
  21. Proc Natl Acad Sci U S A. 1990 Oct;87(19):7507-11 [PMID: 2217181]
  22. Science. 1944 Mar 3;99(2566):183-4 [PMID: 17736901]
  23. Proc Natl Acad Sci U S A. 1989 Aug;86(15):5673-7 [PMID: 2788276]
  24. Science. 1991 Nov 8;254(5033):821-5 [PMID: 1948064]
  25. Gen Comp Endocrinol. 2002 Feb 1;125(2):236-47 [PMID: 11884069]
  26. Gen Comp Endocrinol. 1992 Sep;87(3):385-93 [PMID: 1426941]
  27. Gen Comp Endocrinol. 1985 Oct;60(1):27-34 [PMID: 3932122]
  28. Nature. 1994 Dec 1;372(6505):478-81 [PMID: 7984244]
  29. Gen Comp Endocrinol. 1996 Feb;101(2):139-44 [PMID: 8812353]
  30. J Biol Chem. 1997 Jan 31;272(5):2583-6 [PMID: 9053397]
  31. Fish Physiol Biochem. 1993 Jul;11(1-6):219-32 [PMID: 24202479]
  32. Gen Comp Endocrinol. 1993 Jan;89(1):51-61 [PMID: 8428649]
  33. Nucleic Acids Res. 1995 Jul 11;23(13):2565-6 [PMID: 7630738]
  34. Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998-9002 [PMID: 2461560]
  35. Proc Natl Acad Sci U S A. 1987 Sep;84(18):6434-7 [PMID: 2819877]
  36. J Biol Chem. 1951 Nov;193(1):265-75 [PMID: 14907713]
  37. Anal Biochem. 1987 Apr;162(1):156-9 [PMID: 2440339]
  38. Gene. 1994 Nov 18;149(2):271-6 [PMID: 7959001]
  39. Proc Natl Acad Sci U S A. 1971 Apr;68(4):866-70 [PMID: 5279528]

MeSH Term

Amino Acid Sequence
Animals
Base Sequence
Carps
Cloning, Molecular
DNA Primers
DNA, Complementary
Escherichia coli
Growth Hormone
India
Models, Molecular
Molecular Sequence Data
Nucleic Acid Amplification Techniques
Pituitary Gland
Protein Conformation
Sequence Analysis, DNA

Chemicals

DNA Primers
DNA, Complementary
Growth Hormone
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

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