Analysis of expressed sequence tags from oil palm (Elaeis guineensis).
Stefan Jouannic, Xavier Argout, Frédéric Lechauve, Cécile Fizames, Alain Borgel, Fabienne Morcillo, Frédérique Aberlenc-Bertossi, Yves Duval, James Tregear
Author Information
Stefan Jouannic: IRD/CIRAD Oil Palm Laboratory, Centre IRD Montpellier, France. jouannic@mpl.ird.fr
This is the first report of a systematic study of genes expressed by means of expressed sequence tag (EST) analysis in oil palm, a species of the Arecales order, a phylogenetically key clade of monocotyledons that is not widely represented in the sequence databases. Five different cDNA libraries were generated from male and female inflorescences, shoot apices and zygotic embryos and unidirectional systematic sequencing was performed. A total of 2411 valid EST sequences were thus obtained. Cluster analysis enabled the identification of 209 groups of related sequences and 1874 singletons. Putative functions were assigned to 1252 of the set of 2083 non-redundant ESTs obtained. The EST database described here is a first step towards gene discovery and cDNA array-based expression analysis in oil palm.
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CN600370
Arecaceae
Base Sequence
Cluster Analysis
DNA, Complementary
DNA, Plant
Expressed Sequence Tags
Flowers
Gene Expression Profiling
Gene Library
Molecular Sequence Data
Plant Stems
Seeds
Sequence Analysis, DNA
DNA, Complementary
DNA, Plant
