Immunohistological characterization of macrophage migration inhibitory factor expression in Plasmodium falciparum-infected placentas.
Sujittra Chaisavaneeyakorn, Naomi Lucchi, Carlos Abramowsky, Caroline Othoro, Sansanee C Chaiyaroj, Ya Ping Shi, Bernard L Nahlen, David S Peterson, Julie M Moore, Venkatachalam Udhayakumar
Author Information
Sujittra Chaisavaneeyakorn: Centers for Disease Control and Prevention, 4770 Buford Highway, Mail Stop F-12, Chamblee, GA 30341, USA.
Previously, we have shown that macrophage migration inhibitory factor (MIF) was highly elevated in the placental intervillous blood (IVB) of Plasmodium falciparum-infected women. Here, we compared the expression of MIF in placental tissues obtained from P. falciparum-infected and -uninfected women. Immunoperoxidase staining showed a consistent pattern of MIF expression in syncytiotrophoblasts, extravillous trophoblasts, IVB mononuclear cells, and amniotic epithelial cells, irrespective of their malaria infection status. Cytotrophoblast, villous stroma, and Hofbauer cells showed focal staining. Only amniotic epithelial and IVB mononuclear cells from P. falciparum-infected placentas exhibited significantly higher level of MIF expression than uninfected placentas. Stimulation of syncytilized human trophoblast BeWo cells with P. falciparum-infected erythrocytes that were selected to bind these cells resulted in significant increases in MIF secretion, whereas control erythrocytes, lipopolysaccharides, and synthetic beta-hematin had minimal effect. These findings suggest that placental malaria modulates MIF expression in different placental compartments.
References
Int J STD AIDS. 2000 Jun;11(6):393-401
[PMID: 10872913]
