Distribution of nocardia species in clinical samples and their routine rapid identification in the laboratory.
Georges Wauters, Véronique Avesani, Jacqueline Charlier, Michèle Janssens, Mario Vaneechoutte, Michel Delmée
Author Information
Georges Wauters: Microbiology Unit, University of Louvain, UCL/5490, Av. Hippocrate 54, B-1200 Brussels, Belgium. wauters@mblg.ucl.ac.be
Eighty-six Nocardia strains isolated from clinical samples in Belgium were identified by 16S rRNA gene sequencing. Eighty-three (96%) strains belonged to only six Nocardia species: N. farcinica (38 [44%]), N. nova (19 [22%]), N. cyriacigeorgica (13 [15%]), N. brasiliensis (6 [6.9%]), N. abscessus (5 [5.8%]), and N. paucivorans (2 [2.3%]). A gallery of nine conventional and enzymatic tests was developed for the rapid identification of the most common species isolated during this survey. Pyrrolidonyl aminopeptidase, gamma-glutamyl aminopeptidase, alpha-mannosidase, and alpha-glucosidase were found to be highly discriminating and could be used to develop an identification scheme.
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Algorithms
Bacterial Typing Techniques
Belgium
DNA, Bacterial
DNA, Ribosomal
Genes, rRNA
Humans
Nocardia
Nocardia Infections
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Species Specificity
Time Factors
DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 16S
