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Journal of genetics
Dec, 2007;86 (3): 259-68.

Construction of genetic linkage map of the medicinal and ornamental plant Catharanthus roseus.

Sarika Gupta, Sashi Pandey-Rai, Suchi Srivastava, Subhas Chandra Naithani, Manoj Prasad, Sushil Kumar
Author Information
  1. Sarika Gupta: National Institute for Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, Post Box No. 10531, New Delhi 110 067, India.
PMID: 18305345 DOI: 10.1007/s12041-007-0033-8

Abstract

An integrated genetic linkage map of the medicinal and ornamental plant Catharanthus roseus, based on different types of molecular and morphological markers was constructed, using a F(2) population of 144 plants. The map defines 14 linkage groups (LGs) and consists of 131 marker loci, including 125 molecular DNA markers (76 RAPD, 3 RAPD combinations; 7 ISSR; 2 EST-SSR from Medicago truncatula and 37 other PCR based DNA markers), selected from a total of 472 primers or primer pairs, and six morphological markers (stem pigmentation, leaf lamina pigmentation and shape, leaf petiole and pod size, and petal colour). The total map length is 1131.9 cM (centiMorgans), giving an average map length and distance between two markers equal to 80.9 cM and 8.6 cM, respectively. The morphological markers/genes were found linked with nearest molecular or morphological markers at distances varying from 0.7 to 11.4 cM. Linkage was observed between the morphological markers concerned with lamina shape and petiole size of leaf on LG1 and leaf, stem and petiole pigmentation and pod size on LG8. This is the first genetic linkage map of C. roseus.

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MeSH Term

Base Sequence
Catharanthus
Chromosome Mapping
DNA Primers
DNA, Plant
Expressed Sequence Tags
Genetic Markers
Minisatellite Repeats
Plants, Medicinal
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
Vinca Alkaloids

Chemicals

DNA Primers
DNA, Plant
Genetic Markers
Vinca Alkaloids
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

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