Histological evaluation of gonadal differentiation in fathead minnows (Pimephales promelas).

Cevdet Uguz
Author Information
  1. Cevdet Uguz: Department of Medical Biology and Genetics, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey. cuguz@aku.edu.tr

Abstract

The timing of sex determination and the pattern of sex differentiation have not been studied in fathead minnow even though this species of fish are commonly used as a research model for toxicological studies. In this study, the developmental histology of gonadal development was investigated. Fish were cultured in the laboratory conditions and spawning obtained at a photoperiod of 16 h-light and 8 h-dark. Samples were collected from day 7 fish post-spawning (day 7 fps) to day 150 fps and their gonads were processed for histological examination. Developmental histology was assessed by using a light microscopy. The results showed that ovarian differentiation normally occurs at around day 13 fps, while testicular differentiation normally occurs at around day 22 fps.

MeSH Term

Animals
Cyprinidae
Female
Germ Cells
Gonads
Male
Meiosis
Ovary
Prophase
Sex Differentiation
Testis
Time Factors

Word Cloud

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