Image preprocessing improves Fourier-based texture analysis of nuclear chromatin.

Randall L Adam, Neucimar J Leite, Konradin Metze

Author Information

Randall L Adam: Institute of Computing, Brazil.

PMID: 18630843

OBJECTIVE: To investigate whether preprocessing of digitized images can improve the image analysis of chromatin of cytologic preparations using Fast Fourier Transformation (FFT).
STUDY DESIGN: In a preprocessing step the nuclear borders of the segmented nuclei were smoothed, thus avoiding the Airy ring artifact. We tested this method, comparing the inertia values of digitalized cardiomyocyte nuclei of rats of different ages. Furthermore, we created in silicio nuclear images with chromatin alterations at or nearby the nuclear edge in order to investigate the robustness of our method.
RESULTS: After preprocessing, the FFT-derived variable inertia discriminated significantly better the chromatin structure of the nuclei at different ages in every frequency range. The investigation on simulated nuclei revealed that within the frequency ranges from 1.8 microm to 0.72 microm smoothing of the borders does not interfere with the detection of chromatin changes at the nuclear border.
CONCLUSION: Smoothing of borders in segmented images can improve the analysis of Fourier-derived variables of the chromatin texture.

Animals

Cell Nucleus

Chromatin

Fourier Analysis

Image Processing, Computer-Assisted

Myocytes, Cardiac

Rats

Rats, Wistar

Chromatin

Journal Article

Chromatin texture by computer-assisted image analysis.Preprocessing with image denoising and histogram equalization for endoscopy image analysis using texture analysis.Chromatin texture measurement by Markovian analysis. Use of nuclear models to define and select texture features.Quantitative image analysis of nuclear chromatin distribution for cytological diagnosis.Nuclear texture measurements in image cytometry.Chromatin structure analysis based on a hierarchic texture model.Remote estimation of rice LAI based on Fourier spectrum texture from UAV image.Visual texture perception and Fourier analysis.Fractal-based image texture analysis of trabecular bone architecture.Quantitative assessment of hip osteoarthritis based on image texture analysis.

Chromatin texture is size dependent in follicular adenomas but not in hyperplastic nodules of the thyroid.Molecular characteristics and chromatin texture features in acute promyelocytic leukemia.Fractal dimension of chromatin is an independent prognostic factor for survival in melanoma.

OpenLB
Open Library of Bioscience