Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNAMet.
Hrvoje Lusic, Estella M Gustilo, Franck A P Vendeix, Rob Kaiser, Michael O Delaney, William D Graham, Virginia A Moye, William A Cantara, Paul F Agris, Alexander Deiters
Author Information
Hrvoje Lusic: Department of Chemistry, Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 27695 and Dharmacon, 2650 Crescent Drive #100, Lafayette, CO 80026, USA.
Human mitochondrial methionine transfer RNA (hmtRNA(Met)(CAU)) has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position-34 (f(5)C(34)). The role of this modification in (hmtRNA(Met)(CAU)) for the decoding of AUA, as well as AUG, in both the peptidyl- and aminoacyl-sites of the ribosome in either chain initiation or chain elongation is still unknown. We report the first synthesis and analyses of the tRNA's anticodon stem and loop domain containing the 5-formylcytidine modification. The modification contributes to the tRNA's anticodon domain structure, thermodynamic properties and its ability to bind codons AUA and AUG in translational initiation and elongation.
References
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5428-32
[PMID: 8516284]
