OpenLB
Open Library of Bioscience
Advanced Search
Molecular therapy : the journal of the American Society of Gene Therapy
Apr, 2009;17 (4): 585-92.

Electroporation for the delivery of DNA-based vaccines and immunotherapeutics: current clinical developments.

Angela M Bodles-Brakhop, Richard Heller, Ruxandra Draghia-Akli
Author Information
  1. Angela M Bodles-Brakhop: VGX Pharmaceuticals, Inc., 2700 Research Forest Drive, Suite 180, The Woodlands, Texas 77381, USA. rdraghia@vgxp.com
PMID: 19223870 DOI: 10.1038/mt.2009.5

Abstract

Electroporation (EP) has been used in basic research for the past 25 years to aid in the transfer of DNA into cells in vitro. EP in vivo enhances transfer of DNA vaccines and therapeutic plasmids to the skin, muscle, tumors, and other tissues resulting in high levels of expression, often with serological and clinical benefits. The recent interest in nonviral gene transfer as treatment options for a vast array of conditions has resulted in the refinement and optimization of EP technology. Current research has revealed that EP can be successfully used in many species, including humans. Clinical trials are currently under way. Herein, the transition of EP from basic science to clinical trials will be discussed.

References

  1. Cancer Res. 2002 Oct 1;62(19):5489-94 [PMID: 12359758]
  2. Dev Growth Differ. 2008 Aug;50(6):517-20 [PMID: 18430029]
  3. Expert Opin Biol Ther. 2008 Nov;8(11):1645-57 [PMID: 18847301]
  4. FEBS Lett. 1998 Apr 3;425(3):436-40 [PMID: 9563509]
  5. Hepatology. 2000 Sep;32(3):618-25 [PMID: 10960458]
  6. Mol Ther. 2008 Nov;16(11):1891-7 [PMID: 18714311]
  7. J Transl Med. 2008 Apr 29;6:21 [PMID: 18445282]
  8. Br J Cancer. 2004 Aug 16;91(4):688-94 [PMID: 15280930]
  9. DNA Cell Biol. 2008 Nov;27(11):629-35 [PMID: 18721073]
  10. Mol Ther. 2002 Jun;5(6):668-75 [PMID: 12027550]
  11. Melanoma Res. 2000 Dec;10(6):577-83 [PMID: 11198480]
  12. Cancer Res. 1999 Oct 15;59(20):5059-63 [PMID: 10537273]
  13. J Biomed Sci. 2006 Jul;13(4):481-8 [PMID: 16649071]
  14. Expert Opin Biol Ther. 2007 Nov;7(11):1647-64 [PMID: 17961089]
  15. J Gene Med. 2008 May;10(5):564-74 [PMID: 18302303]
  16. Clin Cancer Res. 2003 Apr;9(4):1284-90 [PMID: 12684396]
  17. Vaccine. 2008 Nov 18;26(49):6225-31 [PMID: 18692108]
  18. Vaccine. 2008 Sep 19;26(40):5210-5 [PMID: 18455277]
  19. Cancer J Sci Am. 2000 Feb;6 Suppl 1:S61-6 [PMID: 10685662]
  20. Expert Opin Biol Ther. 2007 Nov;7(11):1705-21 [PMID: 17961093]
  21. Mol Ther. 2008 May;16(5):862-70 [PMID: 18388931]
  22. Biochem Biophys Res Commun. 2008 May 23;370(1):22-6 [PMID: 18329382]
  23. J Gene Med. 2007 Nov;9(11):986-93 [PMID: 17912759]
  24. Clin Vaccine Immunol. 2008 Jun;15(6):986-94 [PMID: 18400976]
  25. Proc Natl Acad Sci U S A. 2008 Sep 9;105(36):13538-43 [PMID: 18765801]
  26. Curr Gene Ther. 2006 Apr;6(2):243-73 [PMID: 16611045]
  27. Methods Mol Biol. 2008;423:199-214 [PMID: 18370200]
  28. J Clin Invest. 1998 Sep 15;102(6):1258-64 [PMID: 9739060]
  29. Virus Res. 2008 Mar;132(1-2):1-14 [PMID: 18036698]
  30. Drug Deliv. 2007 Jul;14(5):273-7 [PMID: 17613014]
  31. Vaccine. 2008 Jun 13;26(25):3112-20 [PMID: 18430495]
  32. Gene Ther. 2004 Mar;11(6):522-33 [PMID: 14999224]
  33. Nat Biotechnol. 1998 Sep;16(9):867-70 [PMID: 9743122]
  34. Cancer Immun. 2006 Apr 21;6:8 [PMID: 16626110]
  35. FEBS Lett. 1996 Jul 8;389(3):225-8 [PMID: 8766704]
  36. Cancer. 1996 Mar 1;77(5):964-71 [PMID: 8608491]
  37. J Immunol. 2008 Jun 15;180(12):7969-79 [PMID: 18523260]
  38. Mol Ther. 2006 Feb;13(2):320-7 [PMID: 16185933]
  39. Cancer Treat Rev. 2003 Oct;29(5):371-87 [PMID: 12972356]
  40. Methods Mol Biol. 2008;423:19-33 [PMID: 18370188]
  41. Vaccine. 2008 Jul 23;26(31):3947-57 [PMID: 18724414]
  42. Methods Mol Biol. 2008;423:463-72 [PMID: 18370222]
  43. J Immunol. 2007 Oct 1;179(7):4741-53 [PMID: 17878373]
  44. J Virol. 2008 Jun;82(11):5643-9 [PMID: 18353952]
  45. Front Biosci. 2007 Jan 01;12:246-64 [PMID: 17127297]
  46. Methods Mol Biol. 2008;423:259-78 [PMID: 18370205]
  47. J Clin Invest. 2001 Sep;108(5):669-78 [PMID: 11544272]
  48. Curr Opin Mol Ther. 2007 Dec;9(6):554-62 [PMID: 18041666]
  49. Biochem Biophys Res Commun. 2008 Feb 1;366(1):29-35 [PMID: 18036339]
  50. Hum Gene Ther. 2006 Sep;17(9):890-7 [PMID: 16972757]
  51. Virology. 2006 Mar 1;346(1):151-68 [PMID: 16325880]
  52. Cancer Res. 2001 Apr 15;61(8):3281-4 [PMID: 11309280]
  53. Vaccine. 2008 Jan 17;26(3):440-8 [PMID: 18082294]
  54. Curr Opin Mol Ther. 2003 Oct;5(5):463-74 [PMID: 14601514]
  55. J Clin Oncol. 2008 Dec 20;26(36):5896-903 [PMID: 19029422]
  56. J Exp Med. 2008 Jan 21;205(1):7-12 [PMID: 18195078]
  57. J Exp Med. 1999 Dec 6;190(11):1717-22 [PMID: 10587362]
  58. Cancer J Sci Am. 2000 Feb;6 Suppl 1:S67-75 [PMID: 10685663]
  59. C R Acad Sci III. 1991;313(13):613-8 [PMID: 1723647]
  60. Vet Res Commun. 2008 Jun;32(5):341-56 [PMID: 18425596]
  61. DNA Cell Biol. 2003 Dec;22(12):755-63 [PMID: 14683586]
  62. J Virol. 2004 Aug;78(16):8468-76 [PMID: 15280455]
  63. J Immunother. 2005 Jul-Aug;28(4):389-95 [PMID: 16000958]
  64. Mol Biotechnol. 2009 Mar;41(3):286-95 [PMID: 19016008]
  65. Clin Cancer Res. 2006 May 15;12(10):3177-83 [PMID: 16707618]
  66. J Virol. 2008 Jul;82(13):6458-69 [PMID: 18448544]
  67. Methods. 2006 Sep;40(1):86-97 [PMID: 16997717]
  68. Methods Mol Biol. 2008;423:361-72 [PMID: 18370214]
  69. Antivir Ther. 2008;13(4):495-509 [PMID: 18672528]
  70. EMBO J. 1982;1(7):841-5 [PMID: 6329708]
  71. Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1027-32 [PMID: 17209010]
  72. Mol Ther. 2007 Nov;15(11):2044-50 [PMID: 17726460]
  73. Methods Mol Biol. 2008;433:159-75 [PMID: 18679623]
  74. J Immunol. 2006 Nov 15;177(10):7462-71 [PMID: 17082666]
  75. Vaccine. 2006 May 22;24(21):4582-5 [PMID: 16188351]
  76. J Immunol. 1999 Dec 15;163(12):6378-85 [PMID: 10586027]
  77. PLoS One. 2008 Jun 25;3(6):e2517 [PMID: 18575608]
  78. Cancer Res. 1992 Sep 1;52(17):4706-11 [PMID: 1324791]
  79. Vaccine. 2008 Sep 19;26(40):5223-9 [PMID: 18468743]
  80. J Acquir Immune Defic Syndr. 2008 Apr 1;47(4):403-11 [PMID: 18209683]

MeSH Term

Animals
Electroporation
Humans
Immunotherapy
Vaccines, DNA

Chemicals

Vaccines, DNA
Journal Article Research Support, Non-U.S. Gov't Review
Article has an altmetric score of 20

Word Cloud

Created with Highcharts 10.0.0EPtransferclinicalElectroporationusedbasicresearchDNAvaccinestrialspast25yearsaidcellsvitrovivoenhancestherapeuticplasmidsskinmuscletumorstissuesresultinghighlevelsexpressionoftenserologicalbenefitsrecentinterestnonviralgenetreatmentoptionsvastarrayconditionsresultedrefinementoptimizationtechnologyCurrentrevealedcansuccessfullymanyspeciesincludinghumansClinicalcurrentlywayHereintransitionsciencewilldiscusseddeliveryDNA-basedimmunotherapeutics:currentdevelopments

Similar Articles

Cited By