PURPOSE: To determine whether Dixon-based fat separation techniques can provide more robust removal of lipid signals from multiple-mouse magnetic resonance imaging (MRI)-acquired images than conventional frequency selective chemical saturation techniques. MATERIALS AND METHODS: A two-point Dixon technique was implemented using a RARE-based pulse sequence and techniques for multivolume fat suppression were evaluated using a 4-element array of volume resonators at 4.7 T. Images were acquired of both phantoms and mice. RESULTS: Fat saturation was achieved on all four channels of the multiple mouse acquisition with the Dixon technique, while failures of fat saturation were found with chemical saturation techniques. CONCLUSION: This proof of concept study found that Dixon fat separation provided more reliable and homogenous fat suppression than chemical saturation in phantoms and in vivo.
