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Developmental biology
May 01, 2010;341 (1): 176-85.

CACN-1/Cactin interacts genetically with MIG-2 GTPase signaling to control distal tip cell migration in C. elegans.

Hiba Tannoury, Varenka Rodriguez, Ismar Kovacevic, Mouna Ibourk, Myeongwoo Lee, Erin J Cram
Author Information
  1. Hiba Tannoury: Department of Biology, Northeastern University, 360 Huntington Ave, 134 Mugar Hall, Boston, MA 02115, USA.
PMID: 20188721 DOI: 10.1016/j.ydbio.2010.02.025

Abstract

The two specialized C. elegans distal tip cells (DTCs) provide an in vivo model system for the study of developmentally regulated cell migration. We identified cacn-1/cactin, a well-conserved, novel regulator of cell migration in a genome-wide RNAi screen for regulators of DTC migration. RNAi depletion experiments and analysis of the hypomorphic allele cacn-1(tm3126) indicate that CACN-1 is required during DTC migration for proper pathfinding and for cessation of DTC migration at the end of larval morphogenesis. Strong expression of CACN-1 in the DTCs, and data from cell-specific RNAi depletion experiments, suggest that CACN-1 is required cell-autonomously to control DTC migration. Importantly, genetic interaction data with Rac GTPase activators and effectors suggest that CACN-1 acts specifically to inhibit the mig-2/Rac pathway, and in parallel to ced-10/Rac, to control DTC pathfinding.

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Grants

  1. R01 GM085077/NIGMS NIH HHS
  2. R01 GM085077-02/NIGMS NIH HHS
  3. GM085077/NIGMS NIH HHS

MeSH Term

Animals
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Carrier Proteins
Cell Movement
Drosophila Proteins
Gonads
rac GTP-Binding Proteins

Chemicals

Caenorhabditis elegans Proteins
Carrier Proteins
Drosophila Proteins
cactin protein, Drosophila
Mig-2 protein, C elegans
rac GTP-Binding Proteins
Journal Article Research Support, N.I.H., Extramural

Word Cloud

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