The fabrication of low-impedance nanoporous gold multiple-electrode arrays for neural electrophysiology studies.

Erkin Seker, Yevgeny Berdichevsky, Matthew R Begley, Michael L Reed, Kevin J Staley, Martin L Yarmush
Author Information
  1. Erkin Seker: Center for Engineering in Medicine, Department of Surgery, Harvard Medical School, Massachusetts General Hospital, Shriners Hospitals for Children, Boston, MA, USA.

Abstract

Neural electrodes are essential tools for the study of the nervous system and related diseases. Low electrode impedance is a figure of merit for sensitive detection of neural electrical activity and numerous studies have aimed to reduce impedance. Unfortunately, most of these efforts have been tethered by a combination of poor functional coating adhesion, complicated fabrication techniques, and poor fabrication repeatability. We address these issues with a facile method for reliably producing multiple-electrode arrays with low impedance by patterning highly adherent nanoporous gold films using conventional microfabrication techniques. The high surface area-to-volume ratio of self-assembled nanoporous gold results in a more than 25-fold improvement in the electrode-electrolyte impedance, where at 1 kHz, 850 kOmega impedance for conventional Au electrodes is reduced to 30 kOmega for nanoporous gold electrodes. Low impedance provides a superior signal-to-noise ratio for detection of neural activity in noisy environments. We systematically studied the effect of film morphology on electrode impedance and successfully recorded field potentials from rat hippocampal slices. Here, we present our fabrication approach, the relationship between film morphology and impedance, and field potential recordings.

References

  1. Biosens Bioelectron. 2001 Sep;16(7-8):527-33 [PMID: 11544046]
  2. J Neurosci. 2003 Oct 15;23(28):9349-56 [PMID: 14561862]
  3. IEE Proc Nanobiotechnol. 2004 Jun;151(3):109-15 [PMID: 16475852]
  4. J Neurosci. 2007 May 9;27(19):5215-23 [PMID: 17494708]
  5. Biosens Bioelectron. 2006 Jan 15;21(7):1093-100 [PMID: 15961304]
  6. IEEE Trans Biomed Eng. 2004 Jun;51(6):881-9 [PMID: 15188854]
  7. Annu Rev Biomed Eng. 2002;4:407-52 [PMID: 12117764]
  8. Biosens Bioelectron. 2003 Oct 1;18(11):1339-47 [PMID: 12896834]
  9. J Neurosci Methods. 1982 Jan;5(1-2):13-22 [PMID: 7057675]
  10. J Biosci Bioeng. 2005 Aug;100(2):131-43 [PMID: 16198254]
  11. Lab Chip. 2007 Jan;7(1):30-40 [PMID: 17180203]
  12. Biomaterials. 2008 Aug-Sep;29(24-25):3393-9 [PMID: 18501423]
  13. J Neurosci Methods. 2009 Mar 30;178(1):59-64 [PMID: 19100768]
  14. Nature. 2001 Mar 22;410(6827):450-3 [PMID: 11260708]
  15. Biosens Bioelectron. 2004 Sep 15;20(2):358-66 [PMID: 15308242]

Grants

  1. F32-MH079662/NIMH NIH HHS
  2. P41 EB002503-07/NIBIB NIH HHS
  3. F32 MH079662/NIMH NIH HHS
  4. F32 MH079662-01A1/NIMH NIH HHS
  5. P41-EB002503/NIBIB NIH HHS
  6. P41 EB002503/NIBIB NIH HHS

MeSH Term

Animals
Cell Adhesion
Electric Impedance
Electrodes
Electrophysiology
Gold
Hippocampus
Metal Nanoparticles
Microscopy, Electron, Scanning
Neurons
Porosity
Rats
Rats, Sprague-Dawley
Silver

Chemicals

Silver
Gold

Word Cloud

Created with Highcharts 10.0.0impedancefabricationnanoporousgoldelectrodesneuralLowelectrodedetectionactivitystudiespoortechniquesmultiple-electrodearraysconventionalratiokOmegafilmmorphologyfieldNeuralessentialtoolsstudynervoussystemrelateddiseasesfiguremeritsensitiveelectricalnumerousaimedreduceUnfortunatelyeffortstetheredcombinationfunctionalcoatingadhesioncomplicatedrepeatabilityaddressissuesfacilemethodreliablyproducinglowpatterninghighlyadherentfilmsusingmicrofabricationhighsurfacearea-to-volumeself-assembledresults25-foldimprovementelectrode-electrolyte1kHz850Aureduced30providessuperiorsignal-to-noisenoisyenvironmentssystematicallystudiedeffectsuccessfullyrecordedpotentialsrathippocampalslicespresentapproachrelationshippotentialrecordingslow-impedanceelectrophysiology

Similar Articles

Cited By