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Applied and environmental microbiology
Apr, 2010;76 (8): 2681-3.

Heterologous expression of the oxytetracycline biosynthetic pathway in Myxococcus xanthus.

D Cole Stevens, Michael R Henry, Kimberly A Murphy, Christopher N Boddy
Author Information
  1. D Cole Stevens: Department of Chemistry, Syracuse University, Syracuse, New York 132441, USA.
PMID: 20208031 DOI: 10.1128/AEM.02841-09

Abstract

New natural products for drug discovery may be accessed by heterologous expression of bacterial biosynthetic pathways in metagenomic DNA libraries. However, a "universal" host is needed for this experiment. Herein, we show that Myxococcus xanthus is a potential "universal" host for heterologous expression of polyketide biosynthetic gene clusters.

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MeSH Term

Bacterial Proteins
Biosynthetic Pathways
Gene Order
Multigene Family
Myxococcus xanthus
Oxytetracycline
Recombinant Proteins

Chemicals

Bacterial Proteins
Recombinant Proteins
Oxytetracycline
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

Created with Highcharts 10.0.0expressionbiosyntheticheterologous"universal"hostMyxococcusxanthusNewnaturalproductsdrugdiscoverymayaccessedbacterialpathwaysmetagenomicDNAlibrariesHoweverneededexperimentHereinshowpotentialpolyketidegeneclustersHeterologousoxytetracyclinepathway

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