OpenLB
Open Library of Bioscience
Advanced Search
Antimicrobial agents and chemotherapy
Jul, 2010;54 (7): 3007-10.

Minigenome-based reporter system suitable for high-throughput screening of compounds able to inhibit Ebolavirus replication and/or transcription.

Luke D Jasenosky, Gabriele Neumann, Yoshihiro Kawaoka
Author Information
  1. Luke D Jasenosky: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA.
PMID: 20421407 DOI: 10.1128/AAC.00138-10

Abstract

We describe an Ebolavirus minigenome-based system that is suitable for high-throughput screening of compounds able to impair Ebolavirus virus replication and/or transcription. The assay is robust (Z' factor, >0.6) and can be carried out in low-biosafety containment. Results from a pilot screen of 960 compounds are presented.

References

  1. PLoS Pathog. 2008 Nov;4(11):e1000212 [PMID: 19023410]
  2. Toxicol Lett. 2008 Dec 15;183(1-3):105-11 [PMID: 18992795]
  3. Oncol Rep. 2007 Sep;18(3):737-44 [PMID: 17671728]
  4. J Virol. 2004 Jan;78(2):999-1005 [PMID: 14694131]
  5. J Gen Virol. 2001 May;82(Pt 5):1027-1041 [PMID: 11297677]
  6. AIDS Res Hum Retroviruses. 1996 May 1;12(7):569-76 [PMID: 8743082]
  7. J Virol. 1980 Apr;34(1):293-6 [PMID: 6246261]
  8. J Biomol Screen. 1999;4(2):67-73 [PMID: 10838414]
  9. Curr Med Chem Anticancer Agents. 2004 Mar;4(2):149-72 [PMID: 15032720]
  10. Proc Natl Acad Sci U S A. 1966 Dec;56(6):1867-74 [PMID: 16591432]
  11. Chem Biol. 2007 Dec;14(12):1386-92 [PMID: 18096507]
  12. Anticancer Drug Des. 1990 Nov;5(4):307-17 [PMID: 1963303]
  13. J Gen Virol. 1997 Apr;78 ( Pt 4):699-723 [PMID: 9129643]
  14. J Virol. 2002 Jan;76(1):406-10 [PMID: 11739705]
  15. J Virol. 1999 Mar;73(3):2333-42 [PMID: 9971816]
  16. J Virol. 2005 Apr;79(7):4425-33 [PMID: 15767442]
  17. J Gen Microbiol. 1976 Jan;92(1):167-74 [PMID: 1245837]
  18. J Infect Dis. 2007 Nov 15;196 Suppl 2:S136-41 [PMID: 17940941]
  19. Science. 2006 Dec 8;314(5805):1564 [PMID: 17158318]
  20. Toxicol Sci. 2006 Feb;89(2):423-30 [PMID: 16306151]
  21. J Gen Virol. 2001 May;82(Pt 5):1013-1025 [PMID: 11297676]
  22. Nature. 2003 Apr 10;422(6932):611-4 [PMID: 12679788]
  23. Ther Drug Monit. 2000 Oct;22(5):566-73 [PMID: 11034262]
  24. Biochem Biophys Res Commun. 2009 Dec 18;390(3):743-9 [PMID: 19835841]

MeSH Term

Antiviral Agents
Cell Line
Ebolavirus
Genome, Viral
Humans
Transcription, Genetic
Virus Replication

Chemicals

Antiviral Agents
Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.
Article has an altmetric score of 3

Word Cloud

Created with Highcharts 10.0.0Ebolaviruscompoundssystemsuitablehigh-throughputscreeningablereplicationand/ortranscriptiondescribeminigenome-basedimpairvirusassayrobustZ'factor>06cancarriedlow-biosafetycontainmentResultspilotscreen960presentedMinigenome-basedreporterinhibit

Similar Articles

Cited By