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Malaria journal
May 17, 2010;9 131.

Genetic polymorphism of merozoite surface protein-1 and merozoite surface protein-2 in Plasmodium falciparum field isolates from Myanmar.

Jung-Mi Kang, Sung-Ung Moon, Jung-Yeon Kim, Shin-Hyeong Cho, Khin Lin, Woon-Mok Sohn, Tong-Soo Kim, Byoung-Kuk Na
Author Information
  1. Jung-Mi Kang: Department of Parasitology, Brain Korea 21 Biomedical Center, and Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-751, South Korea.
PMID: 20478015 DOI: 10.1186/1475-2875-9-131

Abstract

BACKGROUND: Merozoite surface protein-1 (MSP-1) and MSP-2 of Plasmodium falciparum are potential vaccine candidate antigens for malaria vaccine development. However, extensive genetic polymorphism of the antigens in field isolates of P. falciparum represents a major obstacle for the development of an effective vaccine. In this study, genetic polymorphism of MSP-1 and MSP-2 among P. falciparum field isolates from Myanmar was analysed.
METHODS: A total of 63 P. falciparum infected blood samples, which were collected from patients attending a regional hospital in Mandalay Division, Myanmar, were used in this study. The regions flanking the highly polymorphic characters, block 2 for MSP-1 and block 3 for MSP-2, were genotyped by allele-specific nested-PCR to analyse the population diversity of the parasite. Sequence analysis of the polymorphic regions of MSP-1 and MSP-2 was also conducted to identify allelic diversity in the parasite population.
RESULTS: Diverse allelic polymorphism of MSP-1 and MSP-2 was identified in P. falciparum isolates from Myanmar and most of the infections were determined to be mixed infections. Sequence analysis of MSP-1 block 2 revealed that 14 different alleles for MSP-1 (5 for K1 type and 9 for MAD20 type) were identified. For MSP-2 block 3, a total of 22 alleles (7 for FC27 type and 15 for 3D7 type) were identified.
CONCLUSION: Extensive genetic polymorphism with diverse allele types was identified in MSP-1 and MSP-2 in P. falciparum field isolates from Myanmar. A high level of mixed infections was also observed, as was a high degree of multiplicity of infection.

References

  1. Korean J Parasitol. 2007 Mar;45(1):59-63 [PMID: 17374980]
  2. Parasitol Today. 1994 May;10(5):182-4 [PMID: 15275468]
  3. J Mol Biol. 1987 May 20;195(2):273-87 [PMID: 3079521]
  4. Trop Med Int Health. 2005 Oct;10(10):1060-4 [PMID: 16185241]
  5. Immunol Today. 1991 Mar;12(3):A68-71 [PMID: 2069680]
  6. Malar J. 2003 May 9;2:12 [PMID: 12773207]
  7. Mol Biochem Parasitol. 1994 Aug;66(2):241-8 [PMID: 7808474]
  8. Trans R Soc Trop Med Hyg. 1999 Feb;93 Suppl 1:21-8 [PMID: 10450422]
  9. Mol Microbiol. 2004 Apr;52(1):159-68 [PMID: 15049818]
  10. Trop Med Int Health. 2001 Aug;6(8):607-13 [PMID: 11555426]
  11. Infect Immun. 1999 Nov;67(11):5906-16 [PMID: 10531247]
  12. Am J Trop Med Hyg. 1995 Jan;52(1):81-8 [PMID: 7856831]
  13. Parasitol Today. 1997 Jul;13(7):262-7 [PMID: 15275063]
  14. Infect Genet Evol. 2006 Sep;6(5):417-24 [PMID: 16517218]
  15. Infect Immun. 2006 Feb;74(2):1313-22 [PMID: 16428781]
  16. Exp Parasitol. 2003 Mar-Apr;103(3-4):127-35 [PMID: 12880589]
  17. Malar J. 2010 Jan 01;9:1 [PMID: 20043863]
  18. Malar J. 2008 May 27;7:93 [PMID: 18505558]
  19. Am J Trop Med Hyg. 2004 Jan;70(1):20-6 [PMID: 14971693]
  20. Acta Trop. 2010 May;114(2):71-5 [PMID: 20096258]
  21. Mem Inst Oswaldo Cruz. 1998 Sep-Oct;93(5):631-8 [PMID: 9830530]
  22. Vaccine. 2000 Mar 17;18(18):1925-31 [PMID: 10699342]
  23. Am J Trop Med Hyg. 1999 Dec;61(6):978-85 [PMID: 10674682]
  24. Am J Trop Med Hyg. 1998 Nov;59(5):726-35 [PMID: 9840589]
  25. Acta Trop. 2009 Jan;109(1):30-6 [PMID: 18851938]
  26. Am J Trop Med Hyg. 2002 Dec;67(6):611-6 [PMID: 12518851]
  27. Nat Med. 2000 Jun;6(6):689-92 [PMID: 10835687]
  28. Proc Natl Acad Sci U S A. 2000 Jun 20;97(13):6994-7001 [PMID: 10860962]
  29. Am J Trop Med Hyg. 2004 Mar;70(3):238-44 [PMID: 15031510]
  30. Trans R Soc Trop Med Hyg. 1999 Feb;93 Suppl 1:3-9 [PMID: 10450419]
  31. Trans R Soc Trop Med Hyg. 1999 Jul-Aug;93(4):375-80 [PMID: 10674080]
  32. Mol Biochem Parasitol. 2002 Nov-Dec;125(1-2):163-71 [PMID: 12467983]
  33. Gene. 2006 Jul 19;376(2):224-30 [PMID: 16716539]
  34. J Infect Dis. 2002 Mar 15;185(6):820-7 [PMID: 11920300]
  35. Exp Parasitol. 2007 Jan;115(1):32-40 [PMID: 16797008]
  36. Ann Trop Med Parasitol. 2001 Mar;95(2):117-32 [PMID: 11299119]
  37. Parassitologia. 1999 Sep;41(1-3):409-14 [PMID: 10697894]
  38. Trans R Soc Trop Med Hyg. 1999 Jul-Aug;93(4):369-74 [PMID: 10674079]
  39. Gene. 2003 Jan 30;304:65-75 [PMID: 12568716]

MeSH Term

Alleles
Antigens, Protozoan
Genotype
Humans
Malaria, Falciparum
Merozoite Surface Protein 1
Myanmar
Plasmodium falciparum
Polymerase Chain Reaction
Polymorphism, Genetic
Protozoan Proteins
Sequence Analysis, DNA

Chemicals

Antigens, Protozoan
Merozoite Surface Protein 1
Protozoan Proteins
merozoite surface protein 2, Plasmodium
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 3

Word Cloud

Created with Highcharts 10.0.0MSP-1MSP-2falciparumpolymorphismisolatesPMyanmarfieldblockidentifiedtypesurfacevaccinegeneticinfectionsprotein-1Plasmodiumantigensdevelopmentstudytotalregionspolymorphic23populationdiversityparasiteSequenceanalysisalsoallelicmixedalleleshighmerozoiteBACKGROUND:MerozoitepotentialcandidatemalariaHoweverextensiverepresentsmajorobstacleeffectiveamonganalysedMETHODS:63infectedbloodsamplescollectedpatientsattendingregionalhospitalMandalayDivisionusedflankinghighlycharactersgenotypedallele-specificnested-PCRanalyseconductedidentifyRESULTS:Diversedeterminedrevealed14different5K19MAD20227FC27153D7CONCLUSION:ExtensivediversealleletypeslevelobserveddegreemultiplicityinfectionGeneticprotein-2

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