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The journal of physiological sciences : JPS
Mar, 2011;61 (2): 131-40.

Heat stress enhances mTOR signaling after resistance exercise in human skeletal muscle.

Ryo Kakigi, Hisashi Naito, Yuji Ogura, Hiroyuki Kobayashi, Norio Saga, Noriko Ichinoseki-Sekine, Toshinori Yoshihara, Shizuo Katamoto
Author Information
  1. Ryo Kakigi: Department of Exercise Physiology, Graduate School of Health and Sports Science, Juntendo University, Inzai, Chiba, 270-1695, Japan.
PMID: 21222186 DOI: 10.1007/s12576-010-0130-y

Abstract

This study investigated the effect of heat stress (HS) on mammalian target of rapamycin (mTOR) signaling involved in translation initiation after resistance exercise in human skeletal muscle. Eight young male subjects performed four sets of six maximal repetitions of knee extension exercises, with or without HS, in a randomized crossover design. HS was applied to the belly of the vastus lateralis by using a microwave therapy unit prior to and during exercise. Muscle biopsies were taken from the vastus lateralis before, immediately after, and 1 h after exercise. HS significantly increased the phosphorylation of Akt/PKB, mTOR, and ribosomal protein S6 at 1 h after exercise (P < 0.05), and the 4E-BP1 phosphorylation level, which had initially decreased with exercise, had recovered by 1 h after exercise with HS. In addition, the phosphorylation of ribosomal S6 kinase 1 was significantly increased immediately after exercise with HS (P < 0.05). These results indicate that HS enhances mTOR signaling after resistance exercise in human skeletal muscle.

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MeSH Term

3-Phosphoinositide-Dependent Protein Kinases
Adaptor Proteins, Signal Transducing
Cell Cycle Proteins
Cross-Over Studies
Exercise
Extracellular Signal-Regulated MAP Kinases
Heat-Shock Response
Hot Temperature
Humans
MAP Kinase Signaling System
Male
Muscle, Skeletal
Phosphoproteins
Phosphorylation
Protein Serine-Threonine Kinases
Proto-Oncogene Proteins c-akt
Resistance Training
Ribosomal Protein S6
Ribosomal Protein S6 Kinases, 90-kDa
Signal Transduction
Sirolimus
TOR Serine-Threonine Kinases
Young Adult
p38 Mitogen-Activated Protein Kinases

Chemicals

Adaptor Proteins, Signal Transducing
Cell Cycle Proteins
EIF4EBP1 protein, human
Phosphoproteins
Ribosomal Protein S6
MTOR protein, human
3-Phosphoinositide-Dependent Protein Kinases
Protein Serine-Threonine Kinases
Proto-Oncogene Proteins c-akt
RPS6KA1 protein, human
Ribosomal Protein S6 Kinases, 90-kDa
TOR Serine-Threonine Kinases
Extracellular Signal-Regulated MAP Kinases
p38 Mitogen-Activated Protein Kinases
Sirolimus
Journal Article Randomized Controlled Trial Research Support, Non-U.S. Gov't
Article has an altmetric score of 18

Word Cloud

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