OpenLB
Open Library of Bioscience
Advanced Search
BMC microbiology
Jan 24, 2011;11 18.

Comparison of membrane proteins of Mycobacterium tuberculosis H37Rv and H37Ra strains.

Hiwa Målen, Gustavo A De Souza, Sharad Pathak, Tina Søfteland, Harald G Wiker
Author Information
  1. Hiwa Målen: Section for Microbiology and Immunology, the Gade Institute, University of Bergen, Bergen, Norway.
PMID: 21261938 DOI: 10.1186/1471-2180-11-18

Abstract

BACKGROUND: The potential causes for variation in virulence between distinct M. tuberculosis strains are still not fully known. However, differences in protein expression are probably an important factor. In this study we used a label-free quantitative proteomic approach to estimate differences in protein abundance between two closely related M. tuberculosis strains; the virulent H37Rv strain and its attenuated counterpart H37Ra.
RESULTS: We were able to identify more than 1700 proteins from both strains. As expected, the majority of the identified proteins had similar relative abundance in the two strains. However, 29 membrane-associated proteins were observed with a 5 or more fold difference in their relative abundance in one strain compared to the other. Of note, 19 membrane- and lipo-proteins had higher abundance in H37Rv, while another 10 proteins had a higher abundance in H37Ra. Interestingly, the possible protein-export membrane protein SecF (Rv2586c), and three ABC-transporter proteins (Rv0933, Rv1273c and Rv1819c) were among the more abundant proteins in M. tuberculosis H37Rv.
CONCLUSION: Our data suggests that the bacterial secretion system and the transmembrane transport system may be important determinants of the ability of distinct M. tuberculosis strains to cause disease.

References

  1. J Biol Chem. 2003 Apr 11;278(15):13094-100 [PMID: 12551895]
  2. J Mol Biol. 2007 Sep 14;372(2):422-33 [PMID: 17669421]
  3. Proteomics. 2008 May;8(9):1859-70 [PMID: 18442171]
  4. Mol Cell Proteomics. 2010 Nov;9(11):2414-23 [PMID: 20190197]
  5. Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3963-7 [PMID: 18316738]
  6. J Proteome Res. 2010 Sep 3;9(9):4839-50 [PMID: 20669988]
  7. Mol Microbiol. 1998 Aug;29(3):685-94 [PMID: 9723909]
  8. Infect Immun. 1999 Nov;67(11):5768-74 [PMID: 10531227]
  9. J Proteome Res. 2003 Jan-Feb;2(1):43-50 [PMID: 12643542]
  10. J Bacteriol. 2006 Apr;188(8):2761-73 [PMID: 16585737]
  11. Mol Microbiol. 1998 Feb;27(3):511-8 [PMID: 9489663]
  12. Mol Microbiol. 2003 Apr;48(1):77-84 [PMID: 12657046]
  13. Immunity. 2006 Apr;24(4):351-7 [PMID: 16618591]
  14. Infect Immun. 2001 Sep;69(9):5777-85 [PMID: 11500455]
  15. Cell Host Microbe. 2008 Feb 14;3(2):97-103 [PMID: 18312844]
  16. Tuberculosis (Edinb). 2004;84(3-4):188-96 [PMID: 15207488]
  17. Scand J Immunol. 2007 Aug-Sep;66(2-3):176-91 [PMID: 17635795]
  18. J Bacteriol. 2008 Aug;190(16):5672-80 [PMID: 18567661]
  19. PLoS Pathog. 2008 Feb 8;4(2):e33 [PMID: 18282096]
  20. Science. 1998 Oct 23;282(5389):759-62 [PMID: 9784137]
  21. Tuber Lung Dis. 1999;79(3):153-69 [PMID: 10656114]
  22. J Mol Biol. 1999 Apr 2;287(3):467-84 [PMID: 10092453]
  23. Proteomics. 2007 May;7(10):1702-18 [PMID: 17443846]
  24. Infect Immun. 1998 Feb;66(2):794-9 [PMID: 9453643]
  25. Microbiology (Reading). 2007 Mar;153(Pt 3):652-658 [PMID: 17322184]
  26. J Biosci. 2007 Mar;32(2):185-9 [PMID: 17435310]
  27. Chemotherapy. 2009;55(5):303-7 [PMID: 19556787]
  28. Mol Biol Cell. 2005 Jan;16(1):396-404 [PMID: 15525680]
  29. Microbiology (Reading). 2007 Nov;153(Pt 11):3864-3878 [PMID: 17975095]
  30. Res Microbiol. 1991 Jan;142(1):55-65 [PMID: 1906192]
  31. Nature. 2008 Oct 30;455(7217):1251-4 [PMID: 18820680]
  32. J Biol Chem. 1981 Feb 25;256(4):1604-7 [PMID: 6257680]
  33. Immunity. 2010 Feb 26;32(2):279-89 [PMID: 20171123]
  34. Mol Microbiol. 2003 Sep;49(6):1493-508 [PMID: 12950916]
  35. Infect Immun. 1993 Jul;61(7):2763-73 [PMID: 8514378]
  36. Annu Rev Biochem. 2008;77:643-67 [PMID: 18078384]
  37. PLoS One. 2008 Jun 11;3(6):e2375 [PMID: 18584054]
  38. J Immunol. 2006 Apr 1;176(7):4323-30 [PMID: 16547269]
  39. Mol Microbiol. 1999 Oct;34(2):257-67 [PMID: 10564470]
  40. Antonie Van Leeuwenhoek. 2006 Jul;90(1):93-108 [PMID: 16652204]
  41. Nat Biotechnol. 2008 Dec;26(12):1367-72 [PMID: 19029910]
  42. Infect Immun. 2001 Sep;69(9):5905-7 [PMID: 11500470]
  43. Nat Struct Biol. 2000 Nov;7 Suppl:928-30 [PMID: 11103989]
  44. Infect Immun. 1994 Apr;62(4):1313-9 [PMID: 8132338]
  45. Mol Microbiol. 2004 Jun;52(6):1543-52 [PMID: 15186407]
  46. Proc Natl Acad Sci U S A. 1990 Jun;87(12):4776-80 [PMID: 2112747]
  47. Mol Cell Proteomics. 2005 Dec;4(12):2010-21 [PMID: 16249172]
  48. Genetics. 1996 Feb;142(2):371-81 [PMID: 8852837]
  49. Bioinformatics. 2010 Mar 1;26(5):698-9 [PMID: 20080508]
  50. Mol Cell Proteomics. 2003 Dec;2(12):1284-96 [PMID: 14532352]
  51. Annu Rev Cell Biol. 1992;8:67-113 [PMID: 1282354]
  52. J Mol Evol. 1999 Jan;48(1):22-41 [PMID: 9873074]
  53. Proteomics. 2007 May;7(10):1687-701 [PMID: 17436267]
  54. Nat Rev Microbiol. 2007 Nov;5(11):839-51 [PMID: 17938627]
  55. Mol Microbiol. 1998 Apr;28(1):5-13 [PMID: 9593292]
  56. Mol Microbiol. 1990 Jul;4(7):1207-14 [PMID: 2233255]
  57. J Proteome Res. 2005 May-Jun;4(3):855-61 [PMID: 15952732]
  58. Cell. 1990 Aug 24;62(4):649-57 [PMID: 2167176]
  59. New Microbiol. 2001 Jul;24(3):289-94 [PMID: 11497087]
  60. J Exp Med. 1934 Sep 30;60(4):515-40 [PMID: 19870319]
  61. J Biol Chem. 1996 May 24;271(21):12394-400 [PMID: 8647843]
  62. J Bacteriol. 2000 Jan;182(2):377-84 [PMID: 10629183]
  63. Mol Microbiol. 2006 Apr;60(2):312-30 [PMID: 16573683]

MeSH Term

Bacterial Proteins
Bacterial Secretion Systems
Membrane Proteins
Mycobacterium tuberculosis
Proteomics
Transcription Factors
Transcription, Genetic
Tuberculosis
Virulence

Chemicals

Bacterial Proteins
Bacterial Secretion Systems
Membrane Proteins
Transcription Factors
secF protein, Bacteria
PhoP protein, Bacteria
Comparative Study Journal Article Research Support, Non-U.S. Gov't

Word Cloud

Created with Highcharts 10.0.0proteinsstrainstuberculosisabundanceMH37RvproteinH37RadistinctHoweverdifferencesimportanttwostrainrelativehighermembranesystemBACKGROUND:potentialcausesvariationvirulencestillfullyknownexpressionprobablyfactorstudyusedlabel-freequantitativeproteomicapproachestimatecloselyrelatedvirulentattenuatedcounterpartRESULTS:ableidentify1700expectedmajorityidentifiedsimilar29membrane-associatedobserved5folddifferenceonecomparednote19membrane-lipo-proteinsanother10Interestinglypossibleprotein-exportSecFRv2586cthreeABC-transporterRv0933Rv1273cRv1819camongabundantCONCLUSION:datasuggestsbacterialsecretiontransmembranetransportmaydeterminantsabilitycausediseaseComparisonMycobacterium

Similar Articles

Cited By