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Journal of applied genetics
May, 2011;52 (2): 233-43.

Species diversity of Trichoderma in Poland.

Lidia Błaszczyk, Delfina Popiel, Jerzy Chełkowski, Grzegorz Koczyk, Gary J Samuels, Krzysztof Sobieralski, Marek Siwulski
Author Information
  1. Lidia Błaszczyk: Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland. lgol@igr.poznan.pl
PMID: 21465156 DOI: 10.1007/s13353-011-0039-z

Abstract

In the present study, we reinvestigate the diversity of Trichoderma in Poland utilizing a combination of morphological and molecular/phylogenetic methods. A total of 170 isolates were collected from six different substrata at 49 sites in Poland. These were divided among 14 taxa as follows: 110 of 170 Trichoderma isolates were identified to the species level by the analysis of their ITS1, ITS2 rDNA sequences as: T. harzianum (43 isolates), T. aggressivum (35), T. citrinoviride (11), T. hamatum (9), T. virens (6), T. longibrachiatum (4), T. polysporum (1), and T. tomentosum (1); 60 isolates belonging to the Viride clade were identified based on a fragment of the translation-elongation factor 1-alpha (tef1) gene as: T. atroviride (20 isolates), T. gamsii (2), T. koningii (17), T. viridescens (13), T. viride (7), and T. koningiopsis (1). Identifications were made using the BLAST interface in TrichOKEY and TrichoBLAST ( http://www.isth.info ). The most diverse substrata were soil (nine species per 22 isolates) and decaying wood (nine species per 75 isolates). The most abundant species (25%) isolated from all substrata was T. harzianum.

Associated Data

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MeSH Term

DNA, Ribosomal
DNA, Ribosomal Spacer
Genetic Variation
Molecular Sequence Data
Mycelium
Peptide Elongation Factor Tu
Phylogeny
Phylogeography
Poland
Sequence Analysis, DNA
Trichoderma

Chemicals

DNA, Ribosomal
DNA, Ribosomal Spacer
Peptide Elongation Factor Tu
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

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