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Journal of proteomics
Jun 10, 2011;74 (7): 1036-44.

Proteomic identification of binding targets of isothiocyanates: A perspective on techniques.

Lixin Mi, Zhen Xiao, Timothy D Veenstra, Fung-Lung Chung
Author Information
  1. Lixin Mi: Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, USA. lm293@georgetown.edu
PMID: 21555001 DOI: 10.1016/j.jprot.2011.04.015

Abstract

Intake of cruciferous vegetable is inversely associated with the risk of several cancer types. Isothiocyanates (ITCs) are believed to be important constituents contributing to these cancer-preventive effects. Although several mechanisms, including induction of apoptosis, have been proposed for the anti-carcinogenesis activities of ITCs, detailed upstream triggering events are still not fully understood. Identification of ITC binding targets in cellular proteins is crucial for not only mechanistic studies but also future drug screening and design. In this review, we summarize recent progress in discovery of ITC protein targets from a technical perspective. The advantages and limitations of each method are discussed to facilitate future studies on target discovery of ITCs and perhaps other compounds.

References

  1. Carcinogenesis. 2006 Apr;27(4):782-90 [PMID: 16364922]
  2. Arch Biochem Biophys. 1958 Apr;74(2):443-50 [PMID: 13534673]
  3. Proteomics. 2009 Oct;9(20):4632-41 [PMID: 19743430]
  4. Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11908-13 [PMID: 12193649]
  5. Proc Natl Acad Sci U S A. 2006 Dec 19;103(51):19564-8 [PMID: 17164327]
  6. Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2521-6 [PMID: 10688911]
  7. J Biol Chem. 2009 Jun 19;284(25):17039-17051 [PMID: 19339240]
  8. J Biol Chem. 2008 Aug 8;283(32):22136-46 [PMID: 18524779]
  9. Electrophoresis. 2000 Jul;21(12):2476-86 [PMID: 10939461]
  10. Cancer Res. 2009 Sep 1;69(17):7103-10 [PMID: 19671797]
  11. Bioconjug Chem. 1996 Mar-Apr;7(2):271-3 [PMID: 8983350]
  12. Cancer Res. 2003 Apr 1;63(7):1652-6 [PMID: 12670919]
  13. Mol Cancer Ther. 2004 May;3(5):567-75 [PMID: 15141014]
  14. J Natl Cancer Inst. 2009 Feb 4;101(3):176-93 [PMID: 19176463]
  15. Nat Chem Biol. 2009 Sep;5(9):616-24 [PMID: 19690537]
  16. Antioxid Redox Signal. 2009 May;11(5):981-3 [PMID: 19186997]
  17. J Med Chem. 2011 Feb 10;54(3):809-16 [PMID: 21241062]
  18. BMC Cancer. 2007 Sep 25;7:183 [PMID: 17894894]
  19. Mol Cell Biochem. 2009 Oct;330(1-2):9-22 [PMID: 19363674]
  20. Biol Chem. 2007 Oct;388(10):1069-81 [PMID: 17937621]
  21. Biochemistry. 2009 Oct 20;48(41):9858-70 [PMID: 19737008]
  22. J Biol Chem. 2009 Nov 20;284(47):32425-33 [PMID: 19776019]
  23. Biochem Biophys Res Commun. 2009 Oct 16;388(2):456-62 [PMID: 19682429]
  24. Carcinogenesis. 2011 Feb;32(2):216-23 [PMID: 21109604]
  25. Adv Exp Med Biol. 2001;500:663-73 [PMID: 11765013]
  26. Biochemistry. 1998 Oct 6;37(40):13968-77 [PMID: 9760231]
  27. Cell Struct Funct. 1999 Oct;24(5):329-35 [PMID: 15216890]
  28. Chem Res Toxicol. 2010 Apr 19;23(4):756-65 [PMID: 20131755]
  29. Mutat Res. 2004 Nov 2;555(1-2):191-202 [PMID: 15476860]
  30. Drug Metab Rev. 2000 Aug-Nov;32(3-4):395-411 [PMID: 11139137]
  31. J Biol Chem. 2002 Oct 18;277(42):39334-42 [PMID: 12171915]
  32. Curr Drug Metab. 2002 Jun;3(3):233-55 [PMID: 12083319]
  33. J Biol Chem. 1998 Jul 10;273(28):17940-53 [PMID: 9651401]
  34. Cancer Res. 2007 Jul 1;67(13):6409-16 [PMID: 17616701]
  35. Biochem J. 2009 Oct 12;423(3):315-21 [PMID: 19723024]
  36. Experientia. 1979 Feb 15;35(2):154-5 [PMID: 369877]
  37. Drug Metab Dispos. 1999 Jan;27(1):13-20 [PMID: 9884304]

Grants

  1. R01 CA100853/NCI NIH HHS
  2. R01 CA100853-08/NCI NIH HHS
  3. CA100853/NCI NIH HHS

MeSH Term

Anticarcinogenic Agents
Binding Sites
Chromatography, Affinity
Electrophoresis, Gel, Two-Dimensional
Isothiocyanates
Protein Binding
Protein Conformation
Proteomics
Tandem Mass Spectrometry
Tubulin

Chemicals

Anticarcinogenic Agents
Isothiocyanates
Tubulin
Journal Article Research Support, N.I.H., Extramural Review

Word Cloud

Created with Highcharts 10.0.0ITCstargetsseveralITCbindingstudiesfuturediscoveryperspectiveIntakecruciferousvegetableinverselyassociatedriskcancertypesIsothiocyanatesbelievedimportantconstituentscontributingcancer-preventiveeffectsAlthoughmechanismsincludinginductionapoptosisproposedanti-carcinogenesisactivitiesdetailedupstreamtriggeringeventsstillfullyunderstoodIdentificationcellularproteinscrucialmechanisticalsodrugscreeningdesignreviewsummarizerecentprogressproteintechnicaladvantageslimitationsmethoddiscussedfacilitatetargetperhapscompoundsProteomicidentificationisothiocyanates:techniques

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