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Tissue antigens
Sep, 2011;78 (3): 185-94.

Killer-cell immunoglobulin-like receptor genotyping and HLA killer-cell immunoglobulin-like receptor-ligand identification by real-time polymerase chain reaction.

H A Hong, A S Loubser, D de Assis Rosa, V Naranbhai, W Carr, M Paximadis, D A Lewis, C T Tiemessen, C M Gray
Author Information
  1. H A Hong: AIDS Virus Research Unit, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa.
PMID: 21810083 DOI: 10.1111/j.1399-0039.2011.01749.x

Abstract

The effector function of natural killer (NK) cells is modulated by surface expression of a range of killer-cell immunoglobulin-like receptors (KIRs) that interact with human leukocyte antigen (HLA) class I ligands. We describe the use of real-time polymerase chain reaction (PCR) assays that allow easy and quick detection of 16 KIR genes and the presence/absence of KIR-ligands based on allelic discrimination at codon 80 in the HLA-A/B Bw4 and HLA-C C1/C2 genes. These methods overcome the tedious and expensive nature of conventional KIR genotyping and HLA class I typing using sequence-specific primer (SSP) PCR, sequence-specific oligonucleotide (SSO) hybridization or sequence-based typing (SBT). Using these two cost-effective assays, we measured the frequencies of KIRs, KIR-ligands and KIR/KIR-ligand pairs in a cohort of Black women recruited in South Africa.

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Grants

  1. K01 TW007793/FIC NIH HHS
  2. K01 TW007793-03/FIC NIH HHS
  3. U19 AI051794/NIAID NIH HHS
  4. K01-TW00703-03/FIC NIH HHS

MeSH Term

DNA
DNA Primers
Genotype
HLA Antigens
Humans
Real-Time Polymerase Chain Reaction
Receptors, KIR

Chemicals

DNA Primers
HLA Antigens
Receptors, KIR
DNA
Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't
Article has an altmetric score of 3

Word Cloud

Created with Highcharts 10.0.0immunoglobulin-likeHLAkiller-cellKIRsclassreal-timepolymerasechainreactionPCRassaysKIRgenesKIR-ligandsgenotypingtypingsequence-specificeffectorfunctionnaturalkillerNKcellsmodulatedsurfaceexpressionrangereceptorsinteracthumanleukocyteantigenligandsdescribeusealloweasyquickdetection16presence/absencebasedallelicdiscriminationcodon80HLA-A/BBw4HLA-CC1/C2methodsovercometediousexpensivenatureconventionalusingprimerSSPoligonucleotideSSOhybridizationsequence-basedSBTUsingtwocost-effectivemeasuredfrequenciesKIR/KIR-ligandpairscohortBlackwomenrecruitedSouthAfricaKiller-cellreceptorreceptor-ligandidentification

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