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Microscopy research and technique
Mar, 2012;75 (3): 325-33.

Use of different morphological techniques to analyze the cellular composition of the adult zebrafish optic tectum.

Christopher P Corbo, Nidaa A Othman, Michael C Gutkin, Alejandra Del C Alonso, Zoltan L Fulop
Author Information
  1. Christopher P Corbo: Laboratory for Developmental Brain Research and Neuroplasticity, Department of Biological Sciences, Wagner College, Staten Island, New York 10301, USA.
PMID: 21823204 DOI: 10.1002/jemt.21061

Abstract

Cellular composition of the adult zebrafish (Danio rerio) optic tectal cortex was examined in this study. Morphological techniques such as 1 μm thick serial plastic sections stained with osmium tetroxide and toluidine blue, modified rapid Golgi silver impregnation, GFAP immunohistochemistry, confocal microscopy, as well as scanning and transmission electron microscopy were used. Neuronal and glial components are described and the layers of the cortex are revisited. Specific neuronal arrangements as well as unique glial/ependymal cells are described. A three dimensional rendering of the astrocytic fiber arrangement in the marginal zone is presented and a composite drawing summarizes the cellular composition of the optic tectum.

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Grants

  1. P40 RR012546/NCRR NIH HHS
  2. R15 AG034524/NIA NIH HHS
  3. R15 AG034524-01/NIA NIH HHS
  4. R15AG034524-01/NIA NIH HHS

MeSH Term

Animals
Immunohistochemistry
Microscopy, Confocal
Microscopy, Electron
Superior Colliculi
Zebrafish
Journal Article Research Support, N.I.H., Extramural

Word Cloud

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