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Molecular biology reports
Apr, 2012;39 (4): 4647-53.

EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis.

Hong Cao, Xiaoqian Leng, Chuangju Li, Qiwei Wei, Jianfang Gui, Hanhua Cheng, Rongjia Zhou
Author Information
  1. Hong Cao: Department of Genetics, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
PMID: 21986641 DOI: 10.1007/s11033-011-1256-6

Abstract

Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry, however, a few genes have been identified in this species. We report here construction of a pituitary cDNA library from a 24 years old female Chinese sturgeon just after its spawning, and obtained 2,025 ESTs from the library. 885 unique sequences were identified, which were categorized into 12 functional groups. More than half of the unique sequences (57%) do not match with annotated sequences in the public databases. Three of these novel genes were further identified. Notably, a full-length of cDNA (1,143 bp) encoding somatolactin of 232 amino acids was identified. Phylogenetic analysis showed 97% amino acid identity with White sturgeon somatolactin. RT-PCR analysis indicated that the somatolactin mRNA was only detected in pituitary. Pituitary-specific expression of the somatolactin suggested that the protein may play important physiological functions in pituitary-endocrine system of the Chinese sturgeon.

Associated Data

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EV824675

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MeSH Term

Amino Acid Sequence
Animals
China
DNA, Complementary
Databases, Genetic
Expressed Sequence Tags
Female
Fish Proteins
Fishes
Gene Expression Profiling
Glycoproteins
Molecular Sequence Data
Organ Specificity
Phylogeny
Pituitary Gland
Pituitary Hormones
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment

Chemicals

DNA, Complementary
Fish Proteins
Glycoproteins
Pituitary Hormones
RNA, Messenger
somatolactin protein, fish
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

Created with Highcharts 10.0.0sturgeonsomatolactinChineseidentifiedgenespituitarysequencesAcipensersinensisspeciesimportantcDNAlibraryuniquenovelaminoanalysisrareendangeredalsoresourceaquacultureindustryhoweverreportconstruction24 yearsoldfemalejustspawningobtained2025ESTs885categorized12functionalgroupshalf57%matchannotatedpublicdatabasesThreeNotablyfull-length1143 bpencoding232acidsPhylogeneticshowed97%acididentityWhiteRT-PCRindicatedmRNAdetectedPituitary-specificexpressionsuggestedproteinmayplayphysiologicalfunctionspituitary-endocrinesystemESTdatasetidentification

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