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The Journal of infectious diseases
Nov, 2011;204 Suppl 3 S833-9.

Unconventional secretion of Ebola virus matrix protein VP40.

Olivier Reynard, St Patrick Reid, Audrey Page, Mathieu Mateo, Nathalie Alazard-Dany, Hervé Raoul, Christopher F Basler, Viktor E Volchkov
Author Information
  1. Olivier Reynard: Filovirus Laboratory, Inserm U758, Human Virology Department, Université de Lyon, Ecole Normale Supérieure de Lyon, Lyon, France.
PMID: 21987759 DOI: 10.1093/infdis/jir305

Abstract

The Ebola virus matrix protein VP40 plays an essential role in virus assembly and budding. In this study we reveal that transient VP40 expression results in the release into the culture medium of substantial amounts of soluble monomeric VP40 in addition to the release of virus-like particles containing an oligomeric form of this protein as previously described. We show that VP40 secretion is endoplasmic reticulum/Golgi-independent and is not associated with cell death. Soluble VP40 was observed during Ebola virus infection of cells and was also found in the serum of virus-infected animals albeit in lower amounts. Unconventional secretion of VP40 may therefore play a role in Ebola virus pathogenicity.

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Grants

  1. AI059536/NIAID NIH HHS

MeSH Term

Animals
Chlorocebus aethiops
Culture Media
Ebolavirus
Endoplasmic Reticulum
Gene Expression Regulation, Viral
Golgi Apparatus
HEK293 Cells
Humans
Vero Cells
Viral Matrix Proteins

Chemicals

Culture Media
VP40 protein, virus
Viral Matrix Proteins
Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Word Cloud

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