Cooperation of two bifunctional enzymes in the biosynthesis and attachment of deoxysugars of the antitumor antibiotic mithramycin.
Guojun Wang, Pallab Pahari, Madan K Kharel, Jing Chen, Haining Zhu, Steven G Van Lanen, Jürgen Rohr
Author Information
Guojun Wang: Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, KY 40536, USA.
Two bifunctional enzymes cooperate in the assembly and the positioning of two sugars, D-olivose and D-mycarose, of the anticancer antibiotic mithramycin. MtmC finishes the biosynthesis of both sugar building blocks depending on which MtmGIV activity is supported. MtmGIV transfers these two sugars onto two structurally distinct acceptor substrates. The dual function of these enzymes explains two essential but previously unidentified activities.
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R01 CA091901/NCI NIH HHS
R01 GM105977/NIGMS NIH HHS
Antibiotics, Antineoplastic
Carbohydrate Sequence
Deoxy Sugars
Glycosylation
Glycosyltransferases
Molecular Sequence Data
Plicamycin
Antibiotics, Antineoplastic
Deoxy Sugars
Glycosyltransferases
Plicamycin
