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Applied and environmental microbiology
Dec, 2012;78 (23): 8477-80.

Impact of sorbic acid on germination and outgrowth heterogeneity of Bacillus cereus ATCC 14579 spores.

Heidy M W den Besten, Clint C J van Melis, Jan Willem Sanders, Masja N Nierop Groot, Tjakko Abee
Author Information
  1. Heidy M W den Besten: Wageningen University, Laboratory of Food Microbiology, Wageningen, the Netherlands. Heidy.denBesten@wur.nl
PMID: 23001664 DOI: 10.1128/AEM.02361-12

Abstract

Population heterogeneity complicates the predictability of the outgrowth kinetics of individual spores. Flow cytometry sorting and monitoring of the germination and outgrowth of single dormant spores allowed the quantification of acid-induced spore population heterogeneity at pH 5.5 and in the presence of sorbic acid. This showed that germination efficiency was not a good predictor for heterogeneity in final outgrowth.

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MeSH Term

Bacillus cereus
Culture Media
Flow Cytometry
Hydrogen-Ion Concentration
Sorbic Acid
Spores, Bacterial

Chemicals

Culture Media
Sorbic Acid
Journal Article

Word Cloud

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