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Nature structural & molecular biology
Jul, 2013;20 (7): 884-91.

A bacterial-like mechanism for transcription termination by the Sen1p helicase in budding yeast.

Odil Porrua, Domenico Libri
Author Information
  1. Odil Porrua: Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, Gif sur Yvette, France. porrua-f@cgm.cnrs-gif.fr
PMID: 23748379 DOI: 10.1038/nsmb.2592

Abstract

Transcription termination is essential to generate functional RNAs and to prevent disruptive polymerase collisions resulting from concurrent transcription. The yeast Sen1p helicase is involved in termination of most noncoding RNAs transcribed by RNA polymerase II (RNAPII). However, the mechanism of termination and the role of this protein have remained enigmatic. Here we address the mechanism of Sen1p-dependent termination by using a highly purified in vitro system. We show that Sen1p is the key enzyme of the termination reaction and reveal features of the termination mechanism. Like the bacterial termination factor Rho, Sen1p recognizes the nascent RNA and hydrolyzes ATP to dissociate the elongation complex. Sen1p-dependent termination is highly specific and, notably, does not require the C-terminal domain of RNAPII. We also show that termination is inhibited by RNA-DNA hybrids. Our results elucidate the role of Sen1p in controlling pervasive transcription.

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MeSH Term

Adenosine Triphosphate
Base Sequence
DNA Helicases
DNA-Binding Proteins
Models, Genetic
Molecular Sequence Data
Multiprotein Complexes
Nuclear Proteins
Protein Structure, Tertiary
RNA Helicases
RNA Polymerase II
RNA Precursors
RNA, Fungal
RNA, Small Nucleolar
RNA-Binding Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Species Specificity
Transcription Factors
Transcription Termination, Genetic

Chemicals

DNA-Binding Proteins
Multiprotein Complexes
NAB3 protein, S cerevisiae
NRD1 protein, S cerevisiae
Nuclear Proteins
REB1 protein, S cerevisiae
RNA Precursors
RNA, Fungal
RNA, Small Nucleolar
RNA-Binding Proteins
Saccharomyces cerevisiae Proteins
Transcription Factors
Adenosine Triphosphate
RNA Polymerase II
SEN1 protein, S cerevisiae
DNA Helicases
RNA Helicases
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 5

Word Cloud

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