Biochemical and genotoxic response of naphthalene to fingerlings of milkfish Chanos chanos.
L Palanikumar, A K Kumaraguru, C M Ramakritinan
Author Information
L Palanikumar: Department of Marine and Coastal Studies, School of Energy, Environment and Natural Resources, Madurai Kamaraj University, Madurai, 625021, India. palanikumarl@gmail.com
The present study investigated the acute toxicity, sub-lethal toxicity and biochemical response of naphthalene in fingerlings of milkfish Chanos chanos. The 96 h acute toxicity LC50 values for C. chanos exposed to naphthalene was 5.18 μg l(-1). The estimated no observed effect concentration and lowest observed effect concentration values for naphthalene in C. chanos were 0.42 and 0.69 μg l(-1) respectively for 30 days. The estimated maximum allowable toxicant concentration for naphthalene was 0.53 μg l(-1). Biochemical enzyme markers such as lipid peroxidation, catalase, glutathione S transferase and reduced glutathione were measured in gills and liver tissues of C. chanos exposed to sub-lethal concentrations of naphthalene. Fluctuation in lipid peroxidation and catalase level suggests that naphthalene concentrations play a vital role in induction of oxidative stress in fish. Induction of reduced glutathione level and inhibition of glutathione S-transferase level was observed in naphthalene exposed C. chanos suggesting that there may be enhanced oxidative damage due to free radicals. Increasing concentration increases in number of nuclear abnormalities. The formation of micronuclei and binucleated micronuclei induction by naphthalene confirm its genotoxic potential. The highest levels of DNA damage (% tail length) were observed at 1.24 μg l(-1) of naphthalene. The study suggests that biochemical enzymes, nuclear abnormalities and DNA damage index can serve as a biological marker for naphthalene contamination.
References
Mar Pollut Bull. 2004 Mar;48(5-6):429-33
[PMID: 14980458]
Ecotoxicol Environ Saf. 2002 Feb;51(2):115-27
[PMID: 11886185]
Mutat Res. 2003 Jan 10;534(1-2):65-75
[PMID: 12504755]
Bull Environ Contam Toxicol. 1996 Mar;56(3):425-31
[PMID: 8825965]
Environ Pollut. 2006 Jun;141(3):443-51
[PMID: 16271429]
