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Applied and environmental microbiology
Jan, 2014;80 (2): 714-22.

GouR, a TetR family transcriptional regulator, coordinates the biosynthesis and export of gougerotin in Streptomyces graminearus.

Junhong Wei, Yuqing Tian, Guoqing Niu, Huarong Tan
Author Information
  1. Junhong Wei: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
PMID: 24242236 DOI: 10.1128/AEM.03003-13

Abstract

Gougerotin is a peptidyl nucleoside antibiotic. It functions as a specific inhibitor of protein synthesis by binding ribosomal peptidyl transferase and exhibits a broad spectrum of biological activities. gouR, situated in the gougerotin biosynthetic gene cluster, encodes a TetR family transcriptional regulatory protein. Gene disruption and genetic complementation revealed that gouR plays an important role in the biosynthesis of gougerotin. Transcriptional analysis suggested that GouR represses the transcription of the gouL-to-gouB operon consisting of 11 structural genes and activates the transcription of the major facilitator superfamily (MFS) transporter gene (gouM). Electrophoresis mobility shift assays (EMSAs) and DNase I footprinting experiments showed that GouR has specific DNA-binding activity for the promoter regions of gouL, gouM, and gouR. Our data suggested that GouR modulates gougerotin production by coordinating its biosynthesis and export in Streptomyces graminearus.

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MeSH Term

Bacterial Proteins
Base Sequence
Binding Sites
Deoxyribonuclease I
Electrophoretic Mobility Shift Assay
Gene Expression Regulation, Bacterial
Genetic Complementation Test
Molecular Sequence Data
Multigene Family
Mutation
Operon
Promoter Regions, Genetic
Protein Transport
Pyrimidine Nucleosides
Streptomyces
Trans-Activators

Chemicals

Bacterial Proteins
Pyrimidine Nucleosides
TetR protein, Clostridium tetani
Trans-Activators
gougerotin
Deoxyribonuclease I
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 6

Word Cloud

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