Mitochondrial dysfunction of immortalized human adipose tissue-derived mesenchymal stromal cells from patients with Parkinson's disease.
Hyo Eun Moon, Seung Hee Yoon, Yong Suk Hur, Hyung Woo Park, Ji Young Ha, Kyung-Hee Kim, Jung Hee Shim, Seung Hyun Yoo, Jin H Son, Seung Leal Paek, In Keyoung Kim, Jae Ha Hwang, Dong Gyu Kim, Han-Joon Kim, Beom Seok Jeon, Sung Sup Park, Sun Ha Paek
Author Information
Hyo Eun Moon: Department of Neurosurgery, Seoul National University College of Medicine, Seoul 110-744, Korea. ; Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-744, Korea. ; Ischemic/Hypoxic Disease Institute, Seoul National University College of Medicine, Seoul 110-744, Korea.
Seung Hee Yoon: Department of Brain & Cognitive Sciences, College of Pharmacy, Brain Disease Research Institute, Ewha Woman's University, Seoul 120-750, Korea.
Yong Suk Hur: Department of Biochemistry, Inha University School of Medicine, Incheon 402-751, Korea.
Hyung Woo Park: Department of Neurosurgery, Seoul National University College of Medicine, Seoul 110-744, Korea. ; Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-744, Korea. ; Ischemic/Hypoxic Disease Institute, Seoul National University College of Medicine, Seoul 110-744, Korea.
Ji Young Ha: Department of Brain & Cognitive Sciences, College of Pharmacy, Brain Disease Research Institute, Ewha Woman's University, Seoul 120-750, Korea.
Kyung-Hee Kim: Department of Brain & Cognitive Sciences, College of Pharmacy, Brain Disease Research Institute, Ewha Woman's University, Seoul 120-750, Korea.
Jung Hee Shim: Department of Brain & Cognitive Sciences, College of Pharmacy, Brain Disease Research Institute, Ewha Woman's University, Seoul 120-750, Korea.
Seung Hyun Yoo: Department of Biochemistry, Inha University School of Medicine, Incheon 402-751, Korea.
Jin H Son: Department of Brain & Cognitive Sciences, College of Pharmacy, Brain Disease Research Institute, Ewha Woman's University, Seoul 120-750, Korea.
Seung Leal Paek: Department of Neurosurgery, Seoul National University College of Medicine, Seoul 110-744, Korea. ; Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-744, Korea. ; Ischemic/Hypoxic Disease Institute, Seoul National University College of Medicine, Seoul 110-744, Korea. ; Department of Neurosurgery, Mayo Clinic, USA.
In Keyoung Kim: Department of Neurosurgery, Seoul National University College of Medicine, Seoul 110-744, Korea.
Jae Ha Hwang: Department of Neurosurgery, Seoul National University College of Medicine, Seoul 110-744, Korea.
Dong Gyu Kim: Department of Neurosurgery, Seoul National University College of Medicine, Seoul 110-744, Korea.
Han-Joon Kim: Department of Neurology, Seoul National University College of Medicine, Seoul 110-744, Korea.
Beom Seok Jeon: Department of Neurology, Seoul National University College of Medicine, Seoul 110-744, Korea.
Sung Sup Park: Department of Laboratory Medicine, Seoul National University Hospital, Seoul 110-744, Korea.
Sun Ha Paek: Department of Neurosurgery, Seoul National University College of Medicine, Seoul 110-744, Korea. ; Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-744, Korea. ; Ischemic/Hypoxic Disease Institute, Seoul National University College of Medicine, Seoul 110-744, Korea.
Mitochondrial dysfunction in dopaminergic neurons of patients with idiopathic and familial Parkinson's disease (PD) is well known although the underlying mechanism is not clear. We established a homogeneous population of human adipose tissue-derived mesenchymal stromal cells (hAD-MSCs) from human adult patients with early-onset hereditary familial Parkin-defect PD as well as late-onset idiopathic PD by immortalizing cells with the hTERT gene to better understand the underlying mechanism of PD. The hAD-MSCs from patients with idiopathic PD were designated as "PD", from patients with Parkin-defect PD as "Parkin" and from patients with pituitary adenomas as "non-PD" in short. The pGRN145 plasmid containing hTERT was introduced to establish telomerase immortalized cells. The established hTERT-immortalized cell lines showed chromosomal aneuploidy sustained stably over two-years. The morphological study of mitochondria in the primary and immortalized hAD-MSCs showed that the mitochondria of the non-PD were normal; however, those of the PD and Parkin were gradually damaged. A striking decrease in mitochondrial complex I, II, and IV activities was observed in the hTERT-immortalized cells from the patients with idiopathic and Parkin-defect PD. Comparative Western blot analyses were performed to investigate the expressions of PD specific marker proteins in the hTERT-immortalized cell lines. This study suggests that the hTERT-immortalized hAD-MSC cell lines established from patients with idiopathic and familial Parkin-defect PD could be good cellular models to evaluate mitochondrial dysfunction to better understand the pathogenesis of PD and to develop early diagnostic markers and effective therapy targets for the treatment of PD.
