- Siau Sie Voo: Institute of Biological Chemistry, M.J. Murdock Metabolomics Laboratory, Washington State University, Clark Hall, Room 341, Pullman, WA, 99164-6340, USA.
Many plant natural products are synthesized in specialized cells and tissues. To learn more about metabolism in these cells, they have to be studied in isolation. Here, we describe a protocol for the isolation of epithelial cells that surround secretory cavities in Citrus fruit peel. Cells isolated using laser microdissection are suitable for RNA isolation and downstream transcriptome analyses.