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Endocrinology
Sep, 2014;155 (9): 3315-28.

Rab5 activity regulates GLUT4 sorting into insulin-responsive and non-insulin-responsive endosomal compartments: a potential mechanism for development of insulin resistance.

Kandice L Tessneer, Robert M Jackson, Beth A Griesel, Ann Louise Olson
Author Information
  1. Kandice L Tessneer: Department of Biochemistry and Molecular Biology (K.L.T., R.M.J., B.A.G., A.L.O.), University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73126; and Cardiovascular Biology Program (K.L.T.), Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104.
PMID: 24932807 DOI: 10.1210/en.2013-2148

Abstract

Glucose transporter isoform 4 (GLUT4) is the insulin-responsive glucose transporter mediating glucose uptake in adipose and skeletal muscle. Reduced GLUT4 translocation from intracellular storage compartments to the plasma membrane is a cause of peripheral insulin resistance. Using a chronic hyperinsulinemia (CHI)-induced cell model of insulin resistance and Rab5 mutant overexpression, we determined these manipulations altered endosomal sorting of GLUT4, thus contributing to the development of insulin resistance. We found that CHI induced insulin resistance in 3T3-L1 adipocytes by retaining GLUT4 in a Rab5-activity-dependent compartment that is unable to equilibrate with the cell surface in response to insulin. Furthermore, CHI-mediated retention of GLUT4 in this non-insulin-responsive compartment impaired filling of the transferrin receptor (TfR)-positive and TfR-negative insulin-responsive storage compartments. Our data suggest that hyperinsulinemia may inhibit GLUT4 by chronically maintaining GLUT4 in the Rab5 activity-dependent endosomal pathway and impairing formation of the TfR-negative and TfR-positive insulin-responsive GLUT4 pools. This model suggests that an early event in the development of insulin-resistant glucose transport in adipose tissue is to alter the intracellular localization of GLUT4 to a compartment that does not efficiently equilibrate with the cell surface when insulin levels are elevated for prolonged periods of time.

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Grants

  1. R01 DK081545/NIDDK NIH HHS
  2. R56 DK081545/NIDDK NIH HHS
  3. DK081545/NIDDK NIH HHS

MeSH Term

Adipocytes
Adipose Tissue
Animals
Endosomes
Glucose Transporter Type 4
Humans
Hyperinsulinism
Insulin
Insulin Resistance
Mice
Protein Transport
Receptors, Transferrin
rab5 GTP-Binding Proteins

Chemicals

Glucose Transporter Type 4
Insulin
Receptors, Transferrin
rab5 GTP-Binding Proteins
Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

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