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Head & neck
04, 2016;38 Suppl 1 E459-67.

Novel method of cell line establishment utilizing fluorescence-activated cell sorting resulting in 6 new head and neck squamous cell carcinoma lines.

John Henry Owen, Martin P Graham, Steven B Chinn, Owen F Darr, Douglas B Chepeha, Gregory T Wolf, Carol R Bradford, Thomas E Carey, Mark E P Prince
Author Information
  1. John Henry Owen: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
  2. Martin P Graham: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
  3. Steven B Chinn: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
  4. Owen F Darr: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
  5. Douglas B Chepeha: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
  6. Gregory T Wolf: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
  7. Carol R Bradford: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
  8. Thomas E Carey: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
  9. Mark E P Prince: Department of Otolaryngology/Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
PMID: 25677579 DOI: 10.1002/hed.24019

Abstract

BACKGROUND: The purpose of this study was to present the establishment of new cell lines, which is important to cancer research.
METHODS: Six new head and neck squamous cell carcinoma cell lines were established using a novel fluorescence-activated cell sorting (FACS) method in order to overcome the barrier of fibroblast overgrowth and the susceptibility of primary tumors to fail in vitro.
RESULTS: Antibodies chosen for specific targeting of epithelial cells and fibroblasts successfully separated cells for line establishment in 6 of 12 attempts, providing an alternative method of establishing head and neck squamous cell carcinoma cell lines. Each attempt at cell line establishment resulted in an epithelial carcinoma population, which was genotyped and catalogued as a unique cell line, and a corresponding fibroblast population.
CONCLUSION: The selection of antibody markers could be optimized to aid in the establishment of any cancer cell line derived from any tumor tissue; this method is not limited to head and neck cancer. © 2015 Wiley Periodicals, Inc. Head Neck 38: E459-E467, 2016.

Keywords

cell line fibroblasts flow cytometry squamous cell carcinoma

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Grants

  1. P30 CA046592/NCI NIH HHS
  2. P30 DC005188/NIDCD NIH HHS
  3. P50 CA097248/NCI NIH HHS
  4. R01 DE019126/NIDCR NIH HHS

MeSH Term

Antibodies
Carcinoma, Squamous Cell
Cell Culture Techniques
Cell Line, Tumor
Cell Separation
Epithelial Cells
Fibroblasts
Flow Cytometry
Head and Neck Neoplasms
Humans

Chemicals

Antibodies
Journal Article
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0celllineestablishmentcarcinomalinesheadnecksquamousmethodnewcancerfluorescence-activatedsortingfibroblastepithelialcellsfibroblasts6populationBACKGROUND:purposestudypresentimportantresearchMETHODS:SixestablishedusingnovelFACSorderovercomebarrierovergrowthsusceptibilityprimarytumorsfailvitroRESULTS:Antibodieschosenspecifictargetingsuccessfullyseparated12attemptsprovidingalternativeestablishingattemptresultedgenotypedcatalogueduniquecorrespondingCONCLUSION:selectionantibodymarkersoptimizedaidderivedtumortissuelimited©2015WileyPeriodicalsIncHeadNeck38:E459-E4672016Novelutilizingresultingflowcytometry

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