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Microbes and environments
2015;30 (1): 108-12.

Molecular characterization of viable Legionella spp. in cooling tower water samples by combined use of ethidium monoazide and PCR.

Hiroaki Inoue, Reiko Fujimura, Kunio Agata, Hiroyuki Ohta
Author Information
  1. Hiroaki Inoue: Tsukuba Research Laboratories, Aquas Corporation, 4–4 Midorigahara, Tsukuba, Ibaraki 300–2646, Japan; Ibaraki University College of Agriculture, 3–21–1 Chuou, Ami-machi, Ibaraki 300–0393, Japan.
PMID: 25736979 DOI: 10.1264/jsme2.ME14115

Abstract

Viable Legionella spp. in environmental water samples were characterized phylogenetically by a clone library analysis combining the use of ethidium monoazide and quantitative PCR. To examine the diversity of Legionella spp., six cooling tower water samples and three bath water samples were collected and analyzed. A total of 617 clones were analyzed for their 16S rRNA gene sequences and classified into 99 operational taxonomic units (OTUs). The majority of OTUs were not clustered with currently described Legionella spp., suggesting the wide diversity of not-yet-cultured Legionella groups harbored in cooling tower water environments.

Associated Data

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MeSH Term

Azides
Cluster Analysis
DNA, Ribosomal
Enzyme Inhibitors
Genetic Variation
Legionella
Microbial Viability
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Water Microbiology

Chemicals

Azides
DNA, Ribosomal
Enzyme Inhibitors
RNA, Ribosomal, 16S
8-azidoethidium
Journal Article Research Support, Non-U.S. Gov't

Word Cloud

Created with Highcharts 10.0.0LegionellawatersppsamplescoolingtoweruseethidiummonoazidePCRdiversityanalyzedOTUsViableenvironmentalcharacterizedphylogeneticallyclonelibraryanalysiscombiningquantitativeexaminesixthreebathcollectedtotal617clones16SrRNAgenesequencesclassified99operationaltaxonomicunitsmajorityclusteredcurrentlydescribedsuggestingwidenot-yet-culturedgroupsharboredenvironmentsMolecularcharacterizationviablecombined

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