Single-cell magnetic imaging using a quantum diamond microscope.
D R Glenn, K Lee, H Park, R Weissleder, A Yacoby, M D Lukin, H Lee, R L Walsworth, C B Connolly
Author Information
D R Glenn: Harvard-Smithsonian Center for Astrophysics, Cambridge, Massachusetts, USA.
K Lee: Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
H Park: Department of Physics, Harvard University, Cambridge, Massachusetts, USA.
R Weissleder: Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
A Yacoby: Department of Physics, Harvard University, Cambridge, Massachusetts, USA.
M D Lukin: Department of Physics, Harvard University, Cambridge, Massachusetts, USA.
H Lee: Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
R L Walsworth: Harvard-Smithsonian Center for Astrophysics, Cambridge, Massachusetts, USA.
C B Connolly: Quantum Diamond Technologies, Inc., Somerville, MA, USA.
We apply a quantum diamond microscope for detection and imaging of immunomagnetically labeled cells. This instrument uses nitrogen-vacancy (NV) centers in diamond for correlated magnetic and fluorescence imaging. Our device provides single-cell resolution and a field of view (∼1 mm(2)) two orders of magnitude larger than that of previous NV imaging technologies, enabling practical applications. To illustrate, we quantified cancer biomarkers expressed by rare tumor cells in a large population of healthy cells.
Lab Chip. 2014 Jul 21;14(14):2385-97
[PMID: 24887807 ]
Nat Med. 2009 Nov;15(11):1327-32
[PMID: 19820717 ]
Nature. 2013 Apr 25;496(7446):486-9
[PMID: 23619694 ]
Proc Natl Acad Sci U S A. 2009 Jul 28;106(30):12459-64
[PMID: 19620715 ]
Anal Biochem. 2001 Apr 15;291(2):175-97
[PMID: 11401292 ]
Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20637-40
[PMID: 19074273 ]
Nat Med. 2008 Aug;14(8):869-74
[PMID: 18607350 ]
IEEE J Solid-State Circuits. 2012 Apr;47(4):1056-1064
[PMID: 25308988 ]
Sci Transl Med. 2012 Jul 4;4(141):141ra92
[PMID: 22764208 ]
Biomaterials. 1995 Apr;16(6):483-8
[PMID: 7654876 ]
J Cell Biol. 2011 Feb 7;192(3):373-82
[PMID: 21300848 ]
R33 CA202064/NCI NIH HHS
U54-CA119349/NCI NIH HHS
R01HL113156/NHLBI NIH HHS
U54 CA119349/NCI NIH HHS
R01 HL113156/NHLBI NIH HHS
Antibodies
Biomarkers, Tumor
Cell Line, Tumor
Diagnostic Imaging
Diamond
Humans
Image Processing, Computer-Assisted
MCF-7 Cells
Magnetic Phenomena
Magnetics
Microscopy
Microscopy, Fluorescence
Nanotechnology
Nitrogen
Quantum Theory
Single-Cell Analysis
Antibodies
Biomarkers, Tumor
Diamond
Nitrogen
