Detection of Anaplasma phagocytophilum in Ixodes ricinus ticks from Norway using a realtime PCR assay targeting the Anaplasma citrate synthase gene gltA.
Anna J Henningsson, Dag Hvidsten, Bjørn-Erik Kristiansen, Andreas Matussek, Snorre Stuen, Andrew Jenkins
Author Information
Anna J Henningsson: Department of Clinical Microbiology, Division of Medical Services, County Hospital Ryhov, Jönköping, Sweden. anna.jonsson.henningsson@rjl.se.
Dag Hvidsten: Department of Microbiology and Infection Control, University Hospital of North Norway, Tromsø, Norway. Dag.Hvidsten@unn.no.
Bjørn-Erik Kristiansen: Research group of host-microbe interactions, Department of Medical Biology, Faculty of Health Sciences, UiT - The Arctic University of Norway, Tromsø, Norway. corleoneno@yahoo.no.
Andreas Matussek: Department of Clinical Microbiology, Division of Medical Services, County Hospital Ryhov, Jönköping, Sweden. andreas.matussek@rjl.se.
Snorre Stuen: Department of Production Animal Clinical Sciences, Norwegian University of Life Sciences, Sandnes, Norway. snorre.stuen@nmbu.no.
Andrew Jenkins: Department of Environmental and Health Studies, Telemark University College, Bø, Norway. andrew.jenkins@hit.no.
BACKGROUND: A TaqMan real-time PCR assay targeting the Anaplasma citrate synthase gene, gltA, was developed and used for detection of Anaplasma phagocytophilum in 765 Ixodes ricinus ticks collected from dogs and cats in northern Norway (n = 669) and Telemark county in southern Norway (n = 96). RESULTS: Among the ticks from northern Norway the prevalence of A. phagocytophilum was 3.0 %, while the prevalence in southern Norway was 2.1 % (p = 0.63). The gltA PCR assay showed a high analytical sensitivity (30 genomic units) and efficiency (98.5 %), and its utility in clinical diagnostics should be evaluated in future studies. CONCLUSION: This is the first report of A. phagocytophilum occurrence in ticks collected north of the Arctic Circle in Norway. The prevalence is comparable to that found in Telemark county in southern Norway.
References
J Am Vet Med Assoc. 1969 Jul 15;155(2):462-9
[PMID: 5819585]
