Poly(3-Hydroxybutyrate) Production in Repeated fed-Batch with Cell Recycle Using a Medium with low Carbon Source Concentration.
Jaciane Lutz Ienczak, Mélodi Schmidt, Luci Kelin Quines, Kellen Zanfonato, José Geraldo da Cruz Pradella, Willibaldo Schmidell, Glaucia Maria Falcao de Aragao
Author Information
Jaciane Lutz Ienczak: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), PO box 6192, Campinas, SP, CEP 13083-970, Brazil.
Mélodi Schmidt: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, UFSC, PO Box 476, Florianópolis, SC, CEP 88040-900, Brazil.
Luci Kelin Quines: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, UFSC, PO Box 476, Florianópolis, SC, CEP 88040-900, Brazil.
Kellen Zanfonato: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, UFSC, PO Box 476, Florianópolis, SC, CEP 88040-900, Brazil.
José Geraldo da Cruz Pradella: Laboratório Nacional de Ciência e Tecnologia do Bioetanol (CTBE), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), PO box 6192, Campinas, SP, CEP 13083-970, Brazil.
Willibaldo Schmidell: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, UFSC, PO Box 476, Florianópolis, SC, CEP 88040-900, Brazil.
Glaucia Maria Falcao de Aragao: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, UFSC, PO Box 476, Florianópolis, SC, CEP 88040-900, Brazil. gmf.aragao@gmail.com.
Among approaches applied to obtain high productivity and low production costs in bioprocesses are high cell density and the use of low cost substrates. Usually low cost substrates, as waste/agroindustrial residues, have low carbon concentration, which leads to a difficulty in operating bioprocesses. Real time control of process for intracellular products is also difficult. The present study proposes a strategy of repeated fed-batch with cell recycle to attain high cell density of Cupriavidus necator and high poly(3-hydroxybutyrate) (P(3HB)) productivity, using a substrate with low carbon source concentration (90 g l(-1)). Also, the use of the oxygen uptake rate data was pointed out as an on line solution for process control, once P(3HB) is an intracellular product. The results showed that total biomass (X), residual biomass (Xr) and P(3HB) values at the end of the culture were 61.6 g l(-1), 19.3 g l(-1) and 42.4 g l(-1) respectively, equivalent to 68.8 % of P(3HB) in the cells, and P(3HB) productivity of 1.0 g l(-1) h(-1). Therefore, the strategy proposed was efficient to achieve high productivity and high polymer content from a medium with low carbon source concentration.
