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Journal of plant research
Jan, 2016;129 (1): 51-65.

Cassava root membrane proteome reveals activities during storage root maturation.

Maliwan Naconsie, Manassawe Lertpanyasampatha, Unchera Viboonjun, Supatcharee Netrphan, Masayoshi Kuwano, Naotake Ogasawara, Jarunya Narangajavana
Author Information
  1. Maliwan Naconsie: Deparment of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Rd.,Rajthewee, Phayathai, Bangkok, 10400, Thailand.
  2. Manassawe Lertpanyasampatha: Deparment of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Rd.,Rajthewee, Phayathai, Bangkok, 10400, Thailand.
  3. Unchera Viboonjun: Deparment of Plant Science, Faculty of Science, Mahidol University, Phayathai, Bangkok, 10400, Thailand.
  4. Supatcharee Netrphan: National Center for Genetic Engineering and Biotechnology (BIOTEC), Rangsit, Pathumthani, 10210, Thailand.
  5. Masayoshi Kuwano: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan.
  6. Naotake Ogasawara: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, 630-0192, Japan.
  7. Jarunya Narangajavana: Deparment of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Rd.,Rajthewee, Phayathai, Bangkok, 10400, Thailand. scjnr@mahidol.ac.th.
PMID: 26547558 DOI: 10.1007/s10265-015-0761-4

Abstract

Cassava (Manihot esculenta Crantz) is one of the most important crops of Thailand. Its storage roots are used as food, feed, starch production, and be the important source for biofuel and biodegradable plastic production. Despite the importance of cassava storage roots, little is known about the mechanisms involved in their formation. This present study has focused on comparison of the expression profiles of cassava root proteome at various developmental stages using two-dimensional gel electrophoresis and LC-MS/MS. Based on an anatomical study using Toluidine Blue, the secondary growth was confirmed to be essential during the development of cassava storage root. To investigate biochemical processes occurring during storage root maturation, soluble and membrane proteins were isolated from storage roots harvested from 3-, 6-, 9-, and 12-month-old cassava plants. The proteins with differential expression pattern were analysed and identified to be associated with 8 functional groups: protein folding and degradation, energy, metabolism, secondary metabolism, stress response, transport facilitation, cytoskeleton, and unclassified function. The expression profiling of membrane proteins revealed the proteins involved in protein folding and degradation, energy, and cell structure were highly expressed during early stages of development. Integration of these data along with the information available in genome and transcriptome databases is critical to expand knowledge obtained solely from the field of proteomics. Possible role of identified proteins were discussed in relation with the activities during storage root maturation in cassava.

Keywords

Cassava Membrane protein Proteomic Storage root maturation

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MeSH Term

Chromatography, Liquid
Electrophoresis, Gel, Two-Dimensional
Gene Expression Regulation, Plant
Manihot
Plant Proteins
Plant Roots
Proteome
Sequence Analysis, DNA
Tandem Mass Spectrometry
Transcriptome

Chemicals

Plant Proteins
Proteome
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 2

Word Cloud

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