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Journal of clinical microbiology
Feb, 2016;54 (2): 445-8.

Enhanced Tracking of Nosocomial Transmission of Endemic Sequence Type 22 Methicillin-Resistant Staphylococcus aureus Type IV Isolates among Patients and Environmental Sites by Use of Whole-Genome Sequencing.

Peter M Kinnevey, Anna C Shore, Micheál Mac Aogáin, Eilish Creamer, Gráinne I Brennan, Hilary Humphreys, Thomas R Rogers, Brian O'Connell, David C Coleman
Author Information
  1. Peter M Kinnevey: Microbiology Research Unit, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland.
  2. Anna C Shore: Microbiology Research Unit, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, St. James's Hospital, Dublin, Ireland.
  3. Micheál Mac Aogáin: Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, St. James's Hospital, Dublin, Ireland.
  4. Eilish Creamer: Department of Microbiology, Beaumont Hospital, Dublin, Ireland.
  5. Gráinne I Brennan: Microbiology Research Unit, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland National MRSA Reference Laboratory, St. James's Hospital, Dublin, Ireland.
  6. Hilary Humphreys: Department of Microbiology, Beaumont Hospital, Dublin, Ireland Department of Clinical Microbiology, Royal College of Surgeons in Ireland Education and Research Centre, Beaumont Hospital, Dublin, Ireland.
  7. Thomas R Rogers: Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, St. James's Hospital, Dublin, Ireland.
  8. Brian O'Connell: Department of Clinical Microbiology, School of Medicine, Trinity College Dublin, St. James's Hospital, Dublin, Ireland National MRSA Reference Laboratory, St. James's Hospital, Dublin, Ireland.
  9. David C Coleman: Microbiology Research Unit, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, Dublin, Ireland david.coleman@dental.tcd.ie. ORCID
PMID: 26582829 DOI: 10.1128/JCM.02662-15

Abstract

Whole-genome sequencing (WGS) of 41 patient and environmental sequence type 22 Methicillin-resistant Staphylococcus aureus staphylococcal cassette chromosome mec type IV (ST22-MRSA-IV) isolates recovered over 6 weeks in one acute hospital ward in Dublin, Ireland, where ST22-MRSA IV is endemic, revealed 228 pairwise combinations differing by <40 single nucleotide variants corresponding to potential cross-transmission events (CTEs). In contrast, 15 pairwise combinations of isolates representing five CTEs were previously identified by conventional molecular epidemiological typing. WGS enhanced ST22-MRSA-IV tracking and highlighted potential transmission of MRSA via the hospital environment.

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MeSH Term

Cross Infection
Environmental Microbiology
Genome, Bacterial
High-Throughput Nucleotide Sequencing
Humans
Methicillin-Resistant Staphylococcus aureus
Molecular Epidemiology
Molecular Typing
Staphylococcal Infections
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 1

Word Cloud

Created with Highcharts 10.0.0IVWGStype22StaphylococcusaureusST22-MRSA-IVisolateshospitalpairwisecombinationspotentialCTEsTypeWhole-genomesequencing41patientenvironmentalsequencemethicillin-resistantstaphylococcalcassettechromosomemecrecovered6weeksoneacutewardDublinIrelandST22-MRSAendemicrevealed228differing<40singlenucleotidevariantscorrespondingcross-transmissioneventscontrast15representingfivepreviouslyidentifiedconventionalmolecularepidemiologicaltypingenhancedtrackinghighlightedtransmissionMRSAviaenvironmentEnhancedTrackingNosocomialTransmissionEndemicSequenceMethicillin-ResistantIsolatesamongPatientsEnvironmentalSitesUseWhole-GenomeSequencing

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