Transgenic FingRs for Live Mapping of Synaptic Dynamics in Genetically-Defined Neurons.
Jong-Hyun Son, Matthew D Keefe, Tamara J Stevenson, Joshua P Barrios, Scott Anjewierden, James B Newton, Adam D Douglass, Joshua L Bonkowsky
Author Information
Jong-Hyun Son: Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah.
Matthew D Keefe: Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah.
Tamara J Stevenson: Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah.
Joshua P Barrios: Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah.
Scott Anjewierden: Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah.
James B Newton: Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah.
Adam D Douglass: Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah.
Joshua L Bonkowsky: Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah.
Tools for genetically-determined visualization of synaptic circuits and interactions are necessary to build connectomics of the vertebrate brain and to screen synaptic properties in neurological disease models. Here we develop a transgenic FingR (fibronectin intrabodies generated by mRNA display) technology for monitoring synapses in live zebrafish. We demonstrate FingR labeling of defined excitatory and inhibitory synapses, and show FingR applicability for dissecting synapse dynamics in normal and disease states. Using our system we show that chronic hypoxia, associated with neurological defects in preterm birth, affects dopaminergic neuron synapse number depending on the developmental timing of hypoxia.
Dev Biol. 2011 Apr 15;352(2):393-404
[PMID: 21276790 ]
Synapse. 2007 Mar;61(3):124-37
[PMID: 17146769 ]
Cell. 2014 Jan 30;156(3):522-36
[PMID: 24485458 ]
Mech Dev. 1999 Feb;80(2):153-8
[PMID: 10072782 ]
Dev Dyn. 1995 Jul;203(3):253-310
[PMID: 8589427 ]
BMC Dev Biol. 2008;8:103
[PMID: 18950487 ]
Neuron. 2013 Jun 19;78(6):971-85
[PMID: 23791193 ]
Cell Rep. 2013 Apr 25;3(4):1199-212
[PMID: 23602570 ]
JAMA. 2006 Oct 4;296(13):1602-8
[PMID: 17018805 ]
Elife. 2014;3. doi: 10.7554/eLife.04878
[PMID: 25490154 ]
Pediatrics. 2004 Sep;114(3):805-16
[PMID: 15342857 ]
Dev Dyn. 2011 Oct;240(10):2324-34
[PMID: 21905164 ]
Annu Rev Neurosci. 2001;24:1-29
[PMID: 11283303 ]
Dev Neurobiol. 2012 Mar;72(3):346-57
[PMID: 21309080 ]
Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15729-34
[PMID: 12438650 ]
Nature. 2014 Apr 10;508(7495):207-14
[PMID: 24695228 ]
BMC Biol. 2010;8:126
[PMID: 20920150 ]
PLoS Genet. 2012;8(4):e1002638
[PMID: 22511881 ]
IEEE Pac Vis Symp. 2012;:201-208
[PMID: 23584131 ]
Dev Neurobiol. 2009 Jul;69(8):518-29
[PMID: 19365814 ]
J Neurosci. 1996 May 15;16(10):3166-77
[PMID: 8627355 ]
Nat Neurosci. 2004 Mar;7(3):254-60
[PMID: 14758365 ]
Nat Methods. 2013 Jun;10(6):494-500
[PMID: 23722208 ]
Dev Dyn. 2007 Nov;236(11):3088-99
[PMID: 17937395 ]
DP2 MH100008/NIMH NIH HHS
R21 MH107039/NIMH NIH HHS
DP2 MH100008/NCCDPHP CDC HHS
Animals
Animals, Genetically Modified
Cell Tracking
Fibronectins
Fluorescent Antibody Technique
Gene Expression
Gene Order
Genes, Reporter
Genetic Vectors
Hypoxia
Immunohistochemistry
Neurons
Synapses
Zebrafish
