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The Journal of comparative neurology
Jun 15, 2016;524 (9): 1920-56.

Synaptic circuitry of identified neurons in the antennal lobe of Drosophila melanogaster.

Jürgen Rybak, Giovanni Talarico, Santiago Ruiz, Christopher Arnold, Rafael Cantera, Bill S Hansson
Author Information
  1. Jürgen Rybak: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, 07745, Jena, Germany.
  2. Giovanni Talarico: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, 07745, Jena, Germany.
  3. Santiago Ruiz: Clemente Estable Institute of Biological Research, 11600 Montevideo, Uruguay.
  4. Christopher Arnold: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, 07745, Jena, Germany.
  5. Rafael Cantera: Clemente Estable Institute of Biological Research, 11600 Montevideo, Uruguay.
  6. Bill S Hansson: Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, 07745, Jena, Germany.
PMID: 26780543 DOI: 10.1002/cne.23966

Abstract

In Drosophila melanogaster olfactory sensory neurons (OSNs) establish synapses with projection neurons (PNs) and local interneurons within antennal lobe (AL) glomeruli. Substantial knowledge regarding this circuitry has been obtained by functional studies, whereas ultrastructural evidence of synaptic contacts is scarce. To fill this gap, we studied serial sections of three glomeruli using electron microscopy. Ectopic expression of a membrane-bound peroxidase allowed us to map synaptic sites along PN dendrites. Our data prove for the first time that each of the three major types of AL neurons is both pre- and postsynaptic to the other two types, as previously indicated by functional studies. PN dendrites carry a large proportion of output synapses, with approximately one output per every three input synapses. Detailed reconstructions of PN dendrites showed that these synapses are distributed unevenly, with input and output sites partially segregated along a proximal-distal gradient and the thinnest branches carrying solely input synapses. Moreover, our data indicate synapse clustering, as we found evidence of dendritic tiling of PN dendrites. PN output synapses exhibited T-shaped presynaptic densities, mostly arranged as tetrads. In contrast, output synapses from putative OSNs showed elongated presynaptic densities in which the T-bar platform was supported by several pedestals and contacted as many as 20 postsynaptic profiles. We also discovered synaptic contacts between the putative OSNs. The average synaptic density in the glomerular neuropil was about two synapses/µm(3) . These results are discussed with regard to current models of olfactory glomerular microcircuits across species.

Keywords

Drosophila melanogaster glomerulus olfactory system projection neuron synaptic microcircuits ultrastructure

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MeSH Term

Animals
Animals, Genetically Modified
Arthropod Antennae
Drosophila Proteins
Drosophila melanogaster
Green Fluorescent Proteins
Imaging, Three-Dimensional
Microscopy, Confocal
Microscopy, Electron, Transmission
Models, Anatomic
Neuropil
Synapses

Chemicals

Drosophila Proteins
Green Fluorescent Proteins
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 3

Word Cloud

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