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Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
2016 Jan-Mar;47 (1): 196-201.

Characterization of quinolone resistance in Salmonella spp. isolates from food products and human samples in Brazil.

Bruno Rocha Pribul, Marcia Lima Festivo, Miliane Moreira Soares de Souza, Dalia dos Prazeres Rodrigues
Author Information
  1. Bruno Rocha Pribul: Laboratório de Referência Nacional de Enteroinfecções Bacterianas, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brazil; Laboratório de bacteriologia, Departamento de microbiologia e Imunologia Veterinária, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil. Electronic address: bruno.pribul@ioc.fiocruz.br.
  2. Marcia Lima Festivo: Laboratório de Referência Nacional de Enteroinfecções Bacterianas, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brazil.
  3. Miliane Moreira Soares de Souza: Laboratório de bacteriologia, Departamento de microbiologia e Imunologia Veterinária, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
  4. Dalia dos Prazeres Rodrigues: Laboratório de Referência Nacional de Enteroinfecções Bacterianas, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brazil.
PMID: 26887245 DOI: 10.1016/j.bjm.2015.04.001

Abstract

Non-typhoidal salmonellosis is an important zoonotic disease caused by Salmonella enterica. The aim of this study was to investigate the prevalence of plasmid-mediated quinolone resistance in Salmonella spp. and its association with fluoroquinolone susceptibility in Brazil. A total of 129 NTS isolates (samples from human origin, food from animal origin, environmental, and animal) grouped as from animal (n=62) and human (n=67) food were evaluated between 2009 and 2013. These isolates were investigated through serotyping, antimicrobial susceptibility testing, and the presence of plasmid-mediated quinolone resistance (PMQR) genes (qnr, aac(6')-Ib) and associated integron genes (integrase, and conserved integron region). Resistance to quinolones and/or fluoroquinolones, from first to third generations, was observed. Fifteen isolates were positive for the presence of qnr genes (8 qnrS, 6 qnrB, and 1 qnrD) and twenty three of aac(6')-Ib. The conserved integron region was detected in 67 isolates as variable regions, from ±600 to >1000pb. The spread of NTS involving PMQR carriers is of serious concern and should be carefully monitored.

Keywords

Foodborne diseases Quinolone resistance Salmonella spp.

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MeSH Term

Anti-Bacterial Agents
Brazil
Drug Resistance, Bacterial
Environmental Microbiology
Food Microbiology
Foodborne Diseases
Genes, Bacterial
Humans
Integrons
Microbial Sensitivity Tests
Plasmids
Quinolones
Salmonella Infections
Salmonella enterica
Serotyping

Chemicals

Anti-Bacterial Agents
Quinolones
Journal Article

Word Cloud

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