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The Journal of antibiotics
Nov, 2016;69 (11): 783-790.

Frog skin cultures secrete anti-yellow fever compounds.

Carolina Muñoz-Camargo, Margarita Correa Méndez, Vivian Salazar, Johanna Moscoso, Diana Narváez, Maria Mercedes Torres, Franz Kaston Florez, Helena Groot, Eduardo Mitrani
Author Information
  1. Carolina Muñoz-Camargo: Laboratory of Human Genetics, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.
  2. Margarita Correa Méndez: Laboratory of Human Genetics, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.
  3. Vivian Salazar: Laboratory of Human Genetics, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.
  4. Johanna Moscoso: Laboratory of Human Genetics, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.
  5. Diana Narváez: Laboratory of Human Genetics, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.
  6. Maria Mercedes Torres: Laboratory of Human Genetics, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.
  7. Franz Kaston Florez: Fundación Nativa, Bogotá, Colombia.
  8. Helena Groot: Laboratory of Human Genetics, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia.
  9. Eduardo Mitrani: Institute of Life Sciences, Hebrew University of Jerusalem, Givat Ram, Jerusalem, Israel.
PMID: 27049440 DOI: 10.1038/ja.2016.16

Abstract

There is an urgent need to develop novel antimicrobial substances. Antimicrobial peptides (AMPs) are considered as promising candidates for future therapeutic use. Because of the re-emergence of the Flavivirus infection, and particularly the yellow fever virus (YFV), we have compared the antiviral activities from skin secretions of seven different frog species against YFV (strain 17D). Secretions from Sphaenorhynchus lacteus, Cryptobatrachus boulongeri and Leptodactylus fuscus displayed the more powerful activities. S. lacteus was found to inhibit viral lysis of Vero E6 cells even at the highest viral concentration evaluated of 10 LD. We also report the identification of a novel frenatin-related peptide from S. lacteus and found that this peptide-on its own-can lead to 35% protection against YVF, while displaying no cytotoxicity against somatic cells even at fivefold higher concentrations. These results are attractive and support the need for continued exploration of new sources of AMPs from frog skin secretions such as those described here in the development of new compounds for the treatment of infectious diseases in general and specific viral infections in particular.

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MeSH Term

Amino Acid Sequence
Amphibian Proteins
Animals
Antiviral Agents
Base Sequence
CHO Cells
Chlorocebus aethiops
Cloning, Molecular
Cricetulus
Peptides
Ranidae
Sequence Analysis, DNA
Skin
Vero Cells
Yellow fever virus

Chemicals

Amphibian Proteins
Antiviral Agents
Peptides
Journal Article
Article has an altmetric score of 5

Word Cloud

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