Role of cAMP signalling in winner and loser effects in crayfish agonistic encounters.

Yuto Momohara, Hiroki Minami, Akihiro Kanai, Toshiki Nagayama
Author Information
  1. Yuto Momohara: Division of Biology, Graduate School of Science and Engineering, Yamagata University, 990-8560, Yamagata, Japan.
  2. Hiroki Minami: Division of Biology, Graduate School of Science and Engineering, Yamagata University, 990-8560, Yamagata, Japan.
  3. Akihiro Kanai: Division of Biology, Graduate School of Science and Engineering, Yamagata University, 990-8560, Yamagata, Japan.
  4. Toshiki Nagayama: Department of Biology, Faculty of Science, Yamagata University, Yamagata, Japan.

Abstract

For territorial animals, establishment of status-dependent dominance order is essential to maintain social stability. In agonistic encounters of the crayfish Procambarus clarkii, a difference of body length of 3-7% is enough for larger animals to become dominant. Despite a physical disadvantage, small winners of the first pairings were more likely to win subsequent conflicts with larger inexperienced animals. In contrast, the losers of the first pairings rarely won subsequent conflicts with smaller naive animals. Such experiences of previous winning or losing affected agonistic outcomes for a long period. The winner effects lasted more than 2 weeks and the loser effect lasted about 10 days. Injection of 5HT1 receptor antagonist into the dominant animals 15-30 min after establishment of dominance order blocked the formation of the winner effects. In contrast, injection of adrenergic-like octopamine receptor antagonist into subordinate animals blocked the formation of the loser. 5HT1 receptors are negatively coupled to adenylyl cyclase and adrenergic-like octopamine receptors are positively coupled. Consistent with this, dominant animals failed to show the winner effect when injected with pCPT-cAMP, a cAMP analogue, and subordinate animals failed to show a loser effect when injected with adenylyl cyclase inhibitor SQ 22536. These results suggest that an increase and decrease of cAMP concentration is essential in mediating loser and winner effects, respectively. Furthermore, formation of the loser effect was blocked by injection of protein kinase A (PKA) inhibitor H89, suggesting long-term memory of the loser effect is dependent on the cAMP-PKA signalling pathway.

Keywords

MeSH Term

Adenylyl Cyclase Inhibitors
Animals
Astacoidea
Cyclic AMP
Cyclic AMP-Dependent Protein Kinases
Dominance-Subordination
Ganglia, Invertebrate
Memory, Long-Term
Receptors, Biogenic Amine
Serotonin Antagonists
Signal Transduction

Chemicals

Adenylyl Cyclase Inhibitors
Receptors, Biogenic Amine
Serotonin Antagonists
norsynephrine receptor
Cyclic AMP
Cyclic AMP-Dependent Protein Kinases

Word Cloud

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