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Oxidative medicine and cellular longevity
2016;2016 2761463.

(Z)-5-(2,4-Dihydroxybenzylidene)thiazolidine-2,4-dione Prevents UVB-Induced Melanogenesis and Wrinkle Formation through Suppressing Oxidative Stress in HRM-2 Hairless Mice.

Bonggi Lee, Kyoung Mi Moon, Seong Jin Kim, So Hee Kim, Dae Hyun Kim, Hye Jin An, Ji Won Jeong, Ye Ra Kim, Sujin Son, Min Jo Kim, Ki Wung Chung, Eun Kyeong Lee, Pusoon Chun, Young Mi Ha, Min-Sun Kim, Sang Hyun Mo, Hyung Ryong Moon, Hae Young Chung
Author Information
  1. Bonggi Lee: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
  2. Kyoung Mi Moon: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
  3. Seong Jin Kim: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
  4. So Hee Kim: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
  5. Dae Hyun Kim: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea; Molecular Inflammation Research Center for Ageing Intervention (MRCA), Pusan National University, Republic of Korea.
  6. Hye Jin An: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
  7. Ji Won Jeong: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
  8. Ye Ra Kim: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
  9. Sujin Son: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
  10. Min Jo Kim: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
  11. Ki Wung Chung: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
  12. Eun Kyeong Lee: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
  13. Pusoon Chun: College of Pharmacy, Inje University, Busan 609-735, Republic of Korea.
  14. Young Mi Ha: Department of Chemistry, Dong-A University, Busan 609-735, Republic of Korea.
  15. Min-Sun Kim: College of Pharmacy, Sunchon National University, Sunchon, Republic of Korea.
  16. Sang Hyun Mo: Bio-FD&C, Songdo Mirae-ro 30, Incheon, Republic of Korea.
  17. Hyung Ryong Moon: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea; Molecular Inflammation Research Center for Ageing Intervention (MRCA), Pusan National University, Republic of Korea.
  18. Hae Young Chung: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea; Molecular Inflammation Research Center for Ageing Intervention (MRCA), Pusan National University, Republic of Korea.
PMID: 27242917 DOI: 10.1155/2016/2761463

Abstract

Background. Uncontrolled melanogenesis and wrinkle formation are an indication of photoaging. Our previous studies demonstrated that (Z)-5-(2,4-dihydroxybenzylidene)thiazolidine-2,4-dione (MHY498) inhibited tyrosinase activity and melanogenesis in vitro. Objective. To examine in vivo effects of MHY498 as an antiaging compound on UVB-induced melanogenesis and wrinkle formation, we topically applied MHY498 on dorsal skin of HRM-2 hairless mice. Methods. Using histological analysis, we evaluated effects of MHY498 on melanogenesis and wrinkle formation after UVB exposure. In addition, related molecular signaling pathways were examined using western blotting, fluorometric assay, and enzyme-linked immunosorbent assay. Results. MHY498 suppressed UVB-induced melanogenesis by inhibiting phosphorylation of CREB and translocation of MITF protein into the nucleus, which are key factors for tyrosinase expression. Consistently, tyrosinase protein levels were notably reduced in the dorsal skin of the hairless mice by MHY498 treatment. Furthermore, MHY498 inhibited UVB-induced wrinkle formation and collagen fiber destruction by increasing type 1 procollagen concentration and decreasing protein expression levels of MMPs, which play an essential role in collagen fiber degradation. As a mechanism, MHY498 notably ameliorated UVB-induced oxidative stress and NF-κB activation in the dermal skin of the hairless mice. Conclusion. Our study suggests that MHY498 can be used as a therapeutic or cosmetic agent for preventing uncontrolled melanogenesis and wrinkle formation.

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MeSH Term

Animals
Male
Melanins
Mice
Mice, Hairless
Oxidative Stress
Signal Transduction
Skin
Skin Aging
Thiazolidinediones
Ultraviolet Rays

Chemicals

(Z)-5-(2,4-dihydroxybenzylidene)thiazolidine-2,4-dione
Melanins
Thiazolidinediones
Journal Article

Word Cloud

Created with Highcharts 10.0.0MHY498melanogenesiswrinkleformationUVB-inducedtyrosinaseskinhairlessmiceproteinZ-5-2thiazolidine-24-dioneinhibitedeffectsdorsalHRM-2assayexpressionlevelsnotablycollagenfiberBackgroundUncontrolledindicationphotoagingpreviousstudiesdemonstrated4-dihydroxybenzylideneactivityvitroObjectiveexaminevivoantiagingcompoundtopicallyappliedMethodsUsinghistologicalanalysisevaluatedUVBexposureadditionrelatedmolecularsignalingpathwaysexaminedusingwesternblottingfluorometricenzyme-linkedimmunosorbentResultssuppressedinhibitingphosphorylationCREBtranslocationMITFnucleuskeyfactorsConsistentlyreducedtreatmentFurthermoredestructionincreasingtype1procollagenconcentrationdecreasingMMPsplayessentialroledegradationmechanismamelioratedoxidativestressNF-κBactivationdermalConclusionstudysuggestscanusedtherapeuticcosmeticagentpreventinguncontrolled4-DihydroxybenzylidenePreventsUVB-InducedMelanogenesisWrinkleFormationSuppressingOxidativeStressHairlessMice

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