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Pharmacognosy magazine
May, 2016;12 (Suppl 2): S218-22.

Pyrostegia venusta (Ker Gawl.) Miers Crude Extract and Fractions: Prevention of Dental Biofilm Formation and Immunomodulatory Capacity.

Mayara Brito de Sousa, José Otávio Carrera Silva Júnior, Wagner Luiz Ramos Barbosa, Erika da Silva Valério, Andriele da Mata Lima, Marlon Heggdorne de Araújo, Michelle Frazão Muzitano, Celso Vataru Nakamura, João Carlos Palazzo de Mello, Francisco Martins Teixeira
Author Information
  1. Mayara Brito de Sousa: Department of Post-Graduation Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil.
  2. José Otávio Carrera Silva Júnior: Department of Post-Graduation Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil; Laboratory of Research and Development in Pharmaceutical and Cosmetic, College of Pharmacy, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil.
  3. Wagner Luiz Ramos Barbosa: Department of Post-Graduation Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil.
  4. Erika da Silva Valério: Department of Post-Graduation Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil.
  5. Andriele da Mata Lima: College of Pharmacy, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil.
  6. Marlon Heggdorne de Araújo: Laboratory of Bioactive Products, School of Pharmacy, Federal University of Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiros - IMCT, Alcides da Conceição Street, 159 Novo Cavaleiros, CEP 27933-378, Brazil.
  7. Michelle Frazão Muzitano: Laboratory of Bioactive Products, School of Pharmacy, Federal University of Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiros - IMCT, Alcides da Conceição Street, 159 Novo Cavaleiros, CEP 27933-378, Brazil.
  8. Celso Vataru Nakamura: Department of Post-Graduation Program in Pharmaceutical Sciences, Health Sciences Center, State University of Maringá, Av. Colombo, 5790, Maringá, Paraná CEP 87020-900, Brazil.
  9. João Carlos Palazzo de Mello: Department of Post-Graduation Program in Pharmaceutical Sciences, Health Sciences Center, State University of Maringá, Av. Colombo, 5790, Maringá, Paraná CEP 87020-900, Brazil.
  10. Francisco Martins Teixeira: Department of Post-Graduation Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil; Pharmacy Course, Federal University of Rio de Janeiro, Campus Macaé, Av. Aluízio da Silva Gomes, 50 Granja dos Cavaleiros, CEP 27930-560, Macaé, Rio de Janeiro, CEP 87020-900, Brazil.
PMID: 27279710 DOI: 10.4103/0973-1296.182150

Abstract

BACKGROUND: Caries and periodontal diseases remain as important diseases in the Brazilian population. One important pathogen associated with this situation is Streptococcus mutans and other important factor is this pathogen's ability to adhere firmly to the tooth surface leading to dental biofilm formation and caries development.
OBJECTIVES: Determine the antibacterial and other biological activities of P. venusta related to its potential to be used in the treatment of caries and periodontal disease.
METHODS: The growth inhibition by P. venusta of Streptococcus mutans, S. mitis, S. oralis and Candida albicans was determined using the broth microdilution method. In addition, the effect of the samples in adherence and reducing production of acids by S. mutans, and germ-tube formation of C. albicans was analysed. The Nitric Oxide (NO) production and cytotoxicity of P. venusta to peripheral blood mononuclear cells (PBMC) and RAW 264.7 Cell Line Murine Macrophage from Blood were assessed.
RESULTS: The crude extract (CE) and ethyl-acetate (AF) and n-butanol (BF) fractions showed antibacterial activity. The ethyl-acetate (AF) fraction showed the highest inhibition percentage against the adherence of S. mutans and C. albicans cells without budding, beyond NO production inhibition. There was not any cytotoxicity in the murine macrophages RAW 264.7 cells.
CONCLUSION: Our results suggest that P. venusta presents potential to be used as a preliminary source of compounds that can provide helpful activity when used in prophylaxis or treatment of caries or periodontal disease.
SUMMARY: Biological activities of Pyrostegia venusta and its potential for use in formulations for the prevention of oral diseases. Abbreviations used: NO: Nitric oxide, PBMC: Peripheral blood mononuclear cells, CE: Crude extract, AF: Ethyl-acetate fraction, BF: n-butanol fraction, HF: Hexane fraction, WF: Water fraction, MIC: Minimum inhibitory concentration, MBC: Minimum bactericidal concentration, ATCC: American Type Culture Collection, CFU: Colony-forming units, BHI: Brain heart infusion, RPMI: Roswell Park Memorial Institute, MOPS: 3-(N-morpholino)propanesulfonic acid, DMEM: Dulbecco's modified Eagle's médium, LPS: Lipopolysacharide, MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide, OD: Optical density, AC: Acteoside.

Keywords

Anti-bacterial agents Candida spp. Streptococcus spp caries periodontal disease

References

  1. Mem Inst Oswaldo Cruz. 2002 Oct;97(7):1027-31 [PMID: 12471432]
  2. Arch Oral Biol. 2000 Aug;45(8):639-45 [PMID: 10869475]
  3. Periodontol 2000. 2002;28:12-55 [PMID: 12013340]
  4. PLoS One. 2013;8(2):e56305 [PMID: 23457545]
  5. Br J Pharmacol. 1998 Feb;123(3):353-60 [PMID: 9504374]
  6. J Bacteriol. 1993 Jun;175(11):3247-52 [PMID: 8501028]
  7. Proc Natl Acad Sci U S A. 2005 Apr 12;102(15):5576-81 [PMID: 15800048]
  8. J Ethnopharmacol. 2005 Feb 28;97(2):305-11 [PMID: 15707770]
  9. J Agric Food Chem. 2001 Sep;49(9):4168-70 [PMID: 11559104]
  10. J Antimicrob Chemother. 2006 Nov;58(5):942-9 [PMID: 16973655]
  11. Molecules. 2007 Aug 20;12(8):1950-63 [PMID: 17960098]
  12. Am J Clin Pathol. 1966 Apr;45(4):493-6 [PMID: 5325707]
  13. BMC Complement Altern Med. 2011 Aug 23;11:69 [PMID: 21861910]
  14. Infect Immun. 1981 Apr;32(1):364-72 [PMID: 6452415]
  15. Oral Health Dent Manag. 2013 Jun;12(2):95-104 [PMID: 23756425]
  16. J Ethnopharmacol. 2010 Oct 28;132(1):355-8 [PMID: 20727400]
  17. J Ethnopharmacol. 2012 Jun 14;141(3):1005-11 [PMID: 22504061]
  18. J Clin Exp Dent. 2013 Dec 01;5(5):e279-86 [PMID: 24455095]
  19. Microbiology. 2003 Feb;149(Pt 2):279-94 [PMID: 12624191]
  20. J Immunol Methods. 1986 Nov 6;93(2):157-65 [PMID: 3490518]
  21. J Ethnopharmacol. 2012 Mar 6;140(1):186-92 [PMID: 22265749]
  22. Chem Pharm Bull (Tokyo). 2007 Feb;55(2):223-6 [PMID: 17268092]
  23. J Clin Periodontol. 1995 Jun;22(6):494-500 [PMID: 7560231]
  24. Int J Dent. 2012;2012:719750 [PMID: 22973312]
  25. Trends Microbiol. 2001 Jul;9(7):327-35 [PMID: 11435107]
  26. Clin Chim Acta. 2006 Apr;366(1-2):90-100 [PMID: 16387291]
  27. Indian J Dent Res. 2009 Jan-Mar;20(1):99-106 [PMID: 19336869]
  28. Periodontol 2000. 2000 Oct;24:239-52 [PMID: 11276870]
  29. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002 Mar;93(3):281-6 [PMID: 11925537]
Journal Article

Word Cloud

Created with Highcharts 10.0.0venustafractionperiodontalmutanscariesPScellsdiseasesimportantStreptococcuspotentialuseddiseaseinhibitionalbicansproductionformationantibacterialactivitiestreatmentCandidaadherenceCNitricNOcytotoxicitybloodmononuclearRAW2647extractethyl-acetateAFn-butanolshowedactivityPyrostegiaCrudeMinimumconcentration3-sppBACKGROUND:CariesremainBrazilianpopulationOnepathogenassociatedsituationfactorpathogen'sabilityadherefirmlytoothsurfaceleadingdentalbiofilmdevelopmentOBJECTIVES:DeterminebiologicalrelatedMETHODS:growthmitisoralisdeterminedusingbrothmicrodilutionmethodadditioneffectsamplesreducingacidsgerm-tubeanalysedOxideperipheralPBMCCellLineMurineMacrophageBloodassessedRESULTS:crudeCEBFfractionshighestpercentagewithoutbuddingbeyondmurinemacrophagesCONCLUSION:resultssuggestpresentspreliminarysourcecompoundscanprovidehelpfulprophylaxisSUMMARY:BiologicaluseformulationspreventionoralAbbreviationsused:NO:oxidePBMC:PeripheralCE:AF:Ethyl-acetateBF:HF:HexaneWF:WaterMIC:inhibitoryMBC:bactericidalATCC:AmericanTypeCultureCollectionCFU:Colony-formingunitsBHI:BrainheartinfusionRPMI:RoswellParkMemorialInstituteMOPS:N-morpholinopropanesulfonicacidDMEM:Dulbecco'smodifiedEagle'smédiumLPS:LipopolysacharideMTT:45-Dimethylthiazol-2-yl-25-DiphenyltetrazoliumBromideOD:OpticaldensityAC:ActeosideKerGawlMiersExtractFractions:PreventionDentalBiofilmFormationImmunomodulatoryCapacityAnti-bacterialagents

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