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Frontiers in microbiology
2016;7 817.

Anti-MRSA Activities of Enterocins DD28 and DD93 and Evidences on Their Role in the Inhibition of Biofilm Formation.

Ahmed K Al Atya, Yanath Belguesmia, Gabrielle Chataigne, Rozenn Ravallec, Anne Vachée, Sabine Szunerits, Rabah Boukherroub, Djamel Drider
Author Information
  1. Ahmed K Al Atya: Université de Lille 1 Sciences et Technologies - Institut Charles Viollette Lille, France.
  2. Yanath Belguesmia: Université de Lille 1 Sciences et Technologies - Institut Charles Viollette Lille, France.
  3. Gabrielle Chataigne: Université de Lille 1 Sciences et Technologies - Institut Charles Viollette Lille, France.
  4. Rozenn Ravallec: Université de Lille 1 Sciences et Technologies - Institut Charles Viollette Lille, France.
  5. Anne Vachée: Hôpital Victor Provo de Roubaix Roubaix, France.
  6. Sabine Szunerits: Institut d'Electronique, de Microélectronique et de Nanotechnologie, UMR CNRS 8520, Université Lille 1 Lille, France.
  7. Rabah Boukherroub: Institut d'Electronique, de Microélectronique et de Nanotechnologie, UMR CNRS 8520, Université Lille 1 Lille, France.
  8. Djamel Drider: Université de Lille 1 Sciences et Technologies - Institut Charles Viollette Lille, France.
PMID: 27303396 DOI: 10.3389/fmicb.2016.00817

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) has become a worrisome superbug. This work aimed at studying the effects of two class IIb bacteriocins, enterocins DD28 and DD93 as anti-MRSA agents. Thus, these bacteriocins were purified, from the cultures supernatants of Enterococcus faecalis 28 and 93, using a simplified purification procedure consisting in a cation exchange chromatography and a reversed-phase high-performance liquid chromatography. The anti-Staphylococcal activity was shown in vitro by the assessment of the minimal inhibitory concentration (MIC), followed by a checkerboard and time-kill kinetics experiments. The data unveiled a clear synergistic effect of enterocins DD28 and DD93 in combination with erythromycin or kanamycin against the clinical MRSA-S1 strain. Besides, these combinations impeded as well the MRSA-S1 clinical strain to setup biofilms on stainless steel and glace devices.

Keywords

MRSA antibiotics bacteriocins enterocins synergism

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