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07 07, 2016;6 29344.

LysGH15 kills Staphylococcus aureus without being affected by the humoral immune response or inducing inflammation.

Lei Zhang, Dong Li, Xinwei Li, Liyuan Hu, Mengjun Cheng, Feifei Xia, Pengjuan Gong, Bin Wang, Jinli Ge, Hao Zhang, Ruopeng Cai, Yanmei Wang, Changjiang Sun, Xin Feng, Liancheng Lei, Wenyu Han, Jingmin Gu
Author Information
  1. Lei Zhang: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
  2. Dong Li: Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, 130021, P. R. China.
  3. Xinwei Li: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
  4. Liyuan Hu: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
  5. Mengjun Cheng: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
  6. Feifei Xia: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
  7. Pengjuan Gong: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
  8. Bin Wang: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
  9. Jinli Ge: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
  10. Hao Zhang: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
  11. Ruopeng Cai: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
  12. Yanmei Wang: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
  13. Changjiang Sun: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
  14. Xin Feng: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
  15. Liancheng Lei: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
  16. Wenyu Han: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
  17. Jingmin Gu: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
PMID: 27385518 DOI: 10.1038/srep29344

Abstract

The lysin LysGH15, derived from the staphylococcal phage GH15, exhibits a wide lytic spectrum and highly efficient lytic activity against methicillin-resistant Staphylococcus aureus (MRSA). Here, we found that LysGH15 did not induce resistance in MRSA or methicillin-sensitive S. aureus (MSSA) strains after repeated treatment. Although LysGH15 triggered the generation of LysGH15-specific antibodies in mice, these antibodies did not block lytic activity in vitro (nor the binding capacity of LysGH15). More importantly, when the antibody titre was highest in mice immunized with LysGH15, a single intravenous injection of LysGH15 was sufficient to protect mice against lethal infection with MRSA. These results indicated that LysGH15-specific antibodies did not affect the killing efficiency of LysGH15 against MRSA in vitro or in vivo. LysGH15 also reduced pro-inflammatory cytokines in mice with lethal infections. Furthermore, a high-dose LysGH15 injection did not cause significant adverse effects or pathological changes in the main organs of treated animals. These results provide further evidence for the administration of LysGH15 as an alternative strategy for the treatment of infections caused by MRSA.

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MeSH Term

Animals
Anti-Bacterial Agents
Female
Immunity, Humoral
Inflammation
Methicillin-Resistant Staphylococcus aureus
Mice
Mice, Inbred BALB C
Mucoproteins
Staphylococcal Infections
Staphylococcus Phages

Chemicals

Anti-Bacterial Agents
Mucoproteins
lysin, gastropoda
Journal Article Research Support, Non-U.S. Gov't
Article has an altmetric score of 2

Word Cloud

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